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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
7 α-hydroxy-3-oxo-4-cholestenoic acid in cerebrospinal fl uid refl ects the integrity of the blood-brain barrier
Journal of Lipid Research, Volume 55, No. 2, Year 2014
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Description
Abstract There is a continuous fl ux of the oxysterol 27- hydroxycholesterol (27-OHC) from the circulation across the blood-brain barrier (BBB) into the brain. The major metabolite of 27-OHC in the brain is 7 α -hydroxy-3-oxo-4- cholestenoic acid (7-HOCA). We confirm a recent report describing the presence of this metabolite in cerebrospinal fl uid (CSF) at a relatively high concentration. A simple and accurate method was developed for assay of 7-HOCA in CSF based on isotope dilution-mass spectrometry and use of 2 H 4 -labeled internal standard. The concentration of this metabolite was found to be markedly increased in CSF from patients with a dysfunctional BBB. There was a high correlation between the levels of 7-HOCA in CSF and the CSF/serum albumin ratio. The concentration of 7-HOCA in CSF was not significantly affected by neurodegeneration. Our findings suggest that 7-HOCA could be used as a diagnostic marker for conditions with a dysfunctional BBB. -Saeed, A., F. Floris, U. Andersson, I. Pikuleva, A. LÖvgren-Sandblom, M. Bjerke, M. Paucar, A. Wallin, P. Svenningsson, and I. BjÖrkhem. 7 α -hydroxy-3-oxo-4-cholestenoic acid in cerebrospinal fl uid refl ects the integrity of the blood-brain barrier. Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc..
Authors & Co-Authors
Saeed, Ahmed A.
Sudan, Khartoum
University of Khartoum Faculty of Medicine
Floris, Federico
Unknown Affiliation
Andersson, Ulla
Unknown Affiliation
Pikuleva, Irina A.
United States, Cleveland
Case Western Reserve University
Lövgren-Sandblom, Anita
Unknown Affiliation
Bjerke, Maria
Sweden, Gothenburg
Sahlgrenska Akademin
Paucar, Martin
Sweden, Stockholm
Karolinska Institutet
Wallin, Anders
Unknown Affiliation
Svenningsson, Per
Sweden, Stockholm
Karolinska Institutet
Björkhem, Ingemar
Unknown Affiliation
Statistics
Citations: 32
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1194/jlr.P044982
ISSN:
00222275
e-ISSN:
15397262