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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Genotyping of rubella virus RNA in sera and dried blood spots collected during routine surveillance and in archival sera
Journal of Virological Methods, Volume 187, No. 2, Year 2013
Notification
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Description
Information on the molecular epidemiology of rubella has been valuable in supporting efforts to control and eliminate rubella in several countries. The preferred samples for virus isolation or RNA detection, such as throat swabs, are often not available making it difficult to obtain a robust database of rubella virus sequences. A method for obtaining rubella virus genotypes from more commonly collected samples such as sera or dried blood spots using real-time RT-PCR to screen samples followed by nested set amplification is described. Rubella genotypes were obtained from dried blood spots and recent and archival sera collections. Eighteen percent of the RNAs extracted from the archival sera were real-time RT-PCR positive, and 44% of these RNAs were amplified successfully by nested RT-PCR and sequenced. Implementation of this technique could provide another tool to improve global rubella molecular surveillance. © 2012 .
Authors & Co-Authors
Zheng, Qi
United States, Atlanta
National Center for Immunization and Respiratory Diseases
Abernathy, Emily
United States, Atlanta
National Center for Immunization and Respiratory Diseases
Sun, Hong
United States, Atlanta
National Center for Immunization and Respiratory Diseases
Zhu, Zhen
United States, Atlanta
Centers for Disease Control and Prevention
de Filippis, Ana Maria Bispo
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Akoua-Koffl, Chantal Gnankon
Cote D'ivoire, Abidjan
Institut Pasteur de Cote-d'ivoire
Ahmed, Hinda Jama
Egypt, Cairo
Who Regional Office for the Eastern Mediterranean
Morris-Glasgow, Victoria
Trinidad and Tobago
Paho
Quist-Therson, Margaret
Ghana, Accra
Ghana Health Service
Icenogle, Joseph P.
United States, Atlanta
National Center for Immunization and Respiratory Diseases
Statistics
Citations: 10
Authors: 10
Affiliations: 7
Identifiers
Doi:
10.1016/j.jviromet.2012.11.023
ISSN:
01660934
e-ISSN:
18790984