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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Aqueous normal phase retention of nucleotides on silica hydride-based columns: Method development strategies for analytes revelant in clinical analysis
Journal of Separation Science, Volume 33, No. 6-7, Year 2010
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Description
An aqueous normal phase HPLC method coupled with UV or ESI/MS detection was used for the determination of a wide variety of nucleotides, essential in metabolomics studies. Fifteen nucleotides were tested in clinically relevant mixtures at levels of 100 μg/mL for UV detection and 1 μg/mL for ESI-MS detection. Analysis times for all protocols developed were less than 20 min. The chromatographic conditions were changed to achieve optimized retention and separation of the nucleotides studied. The aqueous normal phase-HPLC methods were developed utilizing two columns, one having a minimally modified hydride surface another having an undecanoic acid moiety on a hydride surface. Volatile, low ionic strength mobile phases were used. Negative ion mode ESI-MS at near neutral pH mobile phase, combined with a TOF detector provided a highly sensitive and specific method, which is equally suitable for quadrupole and ion trap instruments. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Authors & Co-Authors
Matyska, Maria T.
United States, San Jose
San Jose State University
Pesek, Joseph J.
United States, San Jose
San Jose State University
Duley, John A.
Australia, Brisbane
The University of Queensland
Zamzami, Mazin Abdulaziz
Australia, Brisbane
The University of Queensland
Saudi Arabia, Jeddah
King Abdulaziz University
Fischer, Steven M.
United States, Santa Clara
Agilent Technologies
Statistics
Citations: 36
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1002/jssc.200900648
ISSN:
16159306
e-ISSN:
16159314