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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Comparative Transcriptome Analyses of Deltamethrin-Resistant and -Susceptible Anopheles gambiae Mosquitoes from Kenya by RNA-Seq
PLoS ONE, Volume 7, No. 9, Article e44607, Year 2012
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Description
Malaria causes more than 300 million clinical cases and 665,000 deaths each year, and the majority of the mortality and morbidity occurs in sub-Saharan Africa. Due to the lack of effective vaccines and wide-spread resistance to antimalarial drugs, mosquito control is the primary method of malaria prevention and control. Currently, malaria vector control relies on the use of insecticides, primarily pyrethroids. The extensive use of insecticides has imposed strong selection pressures for resistance in the mosquito populations. Consequently, resistance to pyrethroids in Anopheles gambiae, the main malaria vector in sub-Saharan Africa, has become a major obstacle for malaria control. A key element of resistance management is the identification of resistance mechanisms and subsequent development of reliable resistance monitoring tools. Field-derived An. gambiae from Western Kenya were phenotyped as deltamethrin-resistant or -susceptible by the standard WHO tube test, and their expression profile compared by RNA-seq. Based on the current annotation of the An. gambiae genome, a total of 1,093 transcripts were detected as significantly differentially accumulated between deltamethrin-resistant and -susceptible mosquitoes. These transcripts are distributed over the entire genome, with a large number mapping in QTLs previously linked to pyrethorid resistance, and correspond to heat-shock proteins, metabolic and transport functions, signal transduction activities, cytoskeleton and others. The detected differences in transcript accumulation levels between resistant and susceptible mosquitoes reflect transcripts directly or indirectly correlated with pyrethroid resistance. RNA-seq data also were used to perform a de-novo Cufflinks assembly of the An. gambiae genome. © 2012 Bonizzoni et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s002.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s003.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s004.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s005.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s006.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s007.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s008.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3436877/bin/pone.0044607.s009.pdf
Authors & Co-Authors
Bonizzoni, Mariangela
United States, Irvine
University of California, Irvine
Afrane, Yaw Asare
Kenya, Nairobi
Kenya Medical Research Institute
Augustine Dunn, William
Kenya, Nairobi
Kenya Medical Research Institute
United States, Irvine
University of California, Irvine
Atieli, Francis K.
United States, Irvine
University of California, Irvine
Zhou, Goufa
United States, Irvine
University of California, Irvine
Zhong, Daibin
United States, Irvine
University of California, Irvine
Li, Jun
United States, Norman
The University of Oklahoma
Githeko, Andrew K.
Kenya, Nairobi
Kenya Medical Research Institute
Yan, Guiyun Yun
United States, Irvine
University of California, Irvine
Statistics
Citations: 89
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0044607
e-ISSN:
19326203
Research Areas
Infectious Diseases
Noncommunicable Diseases
Study Locations
Kenya