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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Influenza H7N9 and H9N2 viruses: Coexistence in poultry linked to human H7N9 infection and genome characteristics
Journal of Virology, Volume 88, No. 6, Year 2014
Notification
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Description
Avian influenza virus A of the novel H7N9 reassortant subtype was recently found to cause severe human respiratory infections in China. Live poultry markets were suspected locations of the human H7N9 infection sources, based on the cases' exposure histories and sequence similarities between viral isolates. To explore the role of live poultry markets in the origin of the novel H7N9 virus, we systematically examined poultry and environmental specimens from local markets and farms in Hangzhou, using realtime reverse transcription-PCR (RT-PCR) as well as high-throughput next-generation sequencing (NGS). RT-PCR identified specimens positive for the H7 and N9 genomic segments in all of the 12 poultry markets epidemiologically linked to 10 human H7N9 cases. Chickens, ducks, and environmental specimens from the markets contained heavily mixed subtypes, including H7, N9, H9, and N2 and sometimes H5 and N1. The idea of the coexistence of H7N9 and H9N2 subtypes in chickens was further supported by metagenomic sequencing. In contrast, human H7N9 infection cases (n=31) were all negative for H9N2 virus according to real-time RT-PCR. The six internal segments were indistinguishable for the H7N9 and H9N2 viruses. The H9, N2, and internal- segment sequences were very close to the sequence of the H9N2 virus circulating in chickens in China recently. Our results provide direct evidence that H9N2 strains coexisted with the novel human-pathogenic H7N9 influenza virus in epidemiologically linked live poultry markets. Avian influenza A virus of the H9N2 subtype likely made a recent contribution to the evolution of the H7N9 virus and continues to do so. © 2014, American Society for Microbiology.
Authors & Co-Authors
Yu, Xinfen
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Jin, Tao
China, Shenzhen
Bgi-shenzhen
Cui, Yujun
China, Shenzhen
Bgi-shenzhen
China, Beijing
Academy of Military Sciences
Pu, Xiaoying
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Li, Jun
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Xu, Jin
China, Shenzhen
Bgi-shenzhen
Liu, Guang
China, Beijing
University of Chinese Academy of Sciences
Jia, Huijue
China, Shenzhen
Bgi-shenzhen
Liu, Dan
China
Yuhang District Center for Disease Control and Prevention
Song, Shili
China
Yuhang District Center for Disease Control and Prevention
Yu, Yang
China, Hangzhou
The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases
Xie, Li
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Huang, Renjie
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Ding, Hua
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Kou, Yu
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Zhou, Yinyan
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Wang, Yayu
China, Shenzhen
Bgi-shenzhen
Xu, Xun
China, Shenzhen
Bgi-shenzhen
Yin, Ye
China, Shenzhen
Bgi-shenzhen
Wang, Jian
China, Shenzhen
Bgi-shenzhen
Guo, Chenyi
China, Beijing
Academy of Military Sciences
China, Beijing
Consulting Center of Biomedical Statistics
Yang, Xianwei
China, Shenzhen
Bgi-shenzhen
China, Beijing
Academy of Military Sciences
Hu, Liangping
China, Beijing
Consulting Center of Biomedical Statistics
Wu, Xiaopeng
China, Hangzhou
The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases
Wang, Hailong
China, Hangzhou
The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases
Liu, Jun
China, Beijing
Institute of Microbiology Chinese Academy of Sciences
China, Beijing
Chinese Center for Disease Control and Prevention
Zhao, Guoqiu
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Zhou, Jiyong
China, Hangzhou
The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases
Pan, Jingcao
China, Hangzhou
Hangzhou Center for Disease Control and Prevention
Gao, George Fu
China, Beijing
Institute of Microbiology Chinese Academy of Sciences
China, Beijing
Chinese Center for Disease Control and Prevention
Yang, Ruifu
China, Shenzhen
Bgi-shenzhen
China, Beijing
Academy of Military Sciences
Wang, Jun
China, Shenzhen
Bgi-shenzhen
Denmark, Copenhagen
Københavns Universitet
Saudi Arabia, Jeddah
King Abdulaziz University
Statistics
Citations: 94
Authors: 32
Affiliations: 11
Identifiers
Doi:
10.1128/JVI.02059-13
ISSN:
0022538X
e-ISSN:
10985514