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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Prospective monitoring reveals dynamic levels of T cell immunity to mycobacterium tuberculosis in HIV infected individuals
PLoS ONE, Volume 7, No. 6, Article e37920, Year 2012
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Description
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 and CFP-10-specific IFN-γ Elispot assay (RD1-Elispot) on 163 HIV-infected individuals living in a TB-endemic setting. An RD1-Elispot was performed every 3 months for a period of 3-21 months. 62% of RD1-Elispot negative individuals were positive by cultured Elispot. Fluctuations in T cell response were observed with rates of change ranging from -150 to +153 spot-forming cells (SFC)/200,000 PBMC in a 3-month period. To validate these responses we used an RD1-specific real time quantitative PCR assay for monokine-induced by IFN-γ (MIG) and IFN-γ inducible protein-10 (IP10) (MIG: r = 0.6527, p = 0.0114; IP-10: r = 0.6967, p = 0.0056; IP-10+MIG: r = 0.7055, p = 0.0048). During follow-up 30 individuals were placed on ARVs and 4 progressed to active TB. Fluctuations in SFC did not correlate with CD4 count, viral load, treatment initiation, or progression to active TB. The RD1-Elispot appears to have limited value in this setting. © 2012 Mitchell et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3369919/bin/pone.0037920.s001.doc
Authors & Co-Authors
Mitchell, Jessica E.
United States, Cambridge
Massachusetts Institute of Technology
South Africa, Durban
The Nelson R. Mandela Medical School
Chetty, Shivan
South Africa, Durban
The Nelson R. Mandela Medical School
Govender, Pamla
South Africa, Durban
The Nelson R. Mandela Medical School
Pillay, Mona
South Africa, Durban
The Nelson R. Mandela Medical School
Jaggernath, Manjeetha
South Africa, Durban
The Nelson R. Mandela Medical School
Kasmar, Anne G.
United States, Boston
Brigham and Women's Hospital
Ndung'u, Thumbi P.
United States, Cambridge
Massachusetts Institute of Technology
South Africa, Durban
The Nelson R. Mandela Medical School
Klenerman, Paul
United Kingdom, Oxford
Nuffield Department of Medicine
Walker, Bruce D.
United States, Cambridge
Massachusetts Institute of Technology
South Africa, Durban
The Nelson R. Mandela Medical School
Kasprowicz, Victoria O.
United States, Cambridge
Massachusetts Institute of Technology
South Africa, Durban
The Nelson R. Mandela Medical School
Statistics
Citations: 13
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1371/journal.pone.0037920
e-ISSN:
19326203
Research Areas
Infectious Diseases
Study Design
Cohort Study
Study Approach
Quantitative