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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Expression of HYAL1 and Survivin RNA as Diagnostic Molecular Markers for Bladder Cancer
Journal of Urology, Volume 183, No. 2, Year 2010
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Description
Purpose: Urinary tumor markers that help in the early detection of bladder cancer promise a significant improvement in sensitivity, specificity and convenience over conventional, invasive diagnostic tests. We assessed the diagnostic efficacy of hyaluronidase (HYAL1) and survivin for early bladder cancer detection. Materials and Methods: The study included 166 patients diagnosed with bladder carcinoma, 112 with benign bladder lesions and 100 healthy volunteers who served as controls. All underwent serological assessment of schistosomiasis antibody, urine cytology, and hyaluronidase (HYAL1) and survivin RNA estimation by qualitative and semiquantitative reverse transcriptase-polymerase chain reaction in urothelial cells from voided urine. Results: Positivity rates of HYAL1 RNA and survivin RNA on qualitative reverse transcriptase-polymerase chain reaction were significantly different among the 3 groups. Mean rank using semiquantitative method was increased in the malignant vs the other groups. The best cutoff for HYAL1 and survivin RNA was 0.25 each. Using these cutoffs HYAL1 and survivin RNA sensitivity was 91% and 75%, respectively, with absolute specificity. HYAL1 RNA detected all patients with stages 0 and I bladder cancer (p <0.037). Urine cytology sensitivity improved when combined with hyaluronidase or survivin RNA on semiquantitative reverse transcriptase-polymerase chain reaction. Conclusions: The detection of urinary HYAL1 and survivin RNA is a promising noninvasive test for bladder cancer early detection. HYAL1 RNA was more sensitive and specific than urine cytology. Semiquantitative reverse transcriptase-polymerase chain reaction is favored for its high sensitivity and specificity. © 2010 American Urological Association.
Authors & Co-Authors
Eissa, S.
Egypt, Cairo
Ain Shams University
Swellam, Menha M.
Egypt, Giza
National Research Centre
Shehata, Hanan Hussein
Egypt, Cairo
Ain Shams University
El-Khouly, Inas M.
Egypt, Cairo
Ain Shams University
El-Zayat, Tarek Mostafa
Egypt, Cairo
Faculty of Medicine - Ain Shams University
El-Ahmady, Omar E.
Egypt, Cairo
Faculty of Pharmacy
Statistics
Citations: 43
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/j.juro.2009.10.024
ISSN:
00225347
Research Areas
Cancer
Infectious Diseases
Study Approach
Qualitative