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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Sputum Mycobacterium tuberculosis mRNA as a marker of bacteriologic clearance in response to antituberculosis therapy
Journal of Clinical Microbiology, Volume 48, No. 1, Year 2010
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Description
mRNA is a marker of cell viability. Quantifying Mycobacterium tuberculosis mRNA in sputum is a promising tool for monitoring response to antituberculosis therapy and evaluating the efficacy of individual drugs. mRNA levels were measured in sputum specimens from patients with tuberculosis (TB) receiving monotherapy in an early bactericidal activity study of fluoroquinolones and in those receiving a standard rifampin-based regimen in an interleukin-2 (IL-2) trial. In the early bactericidal activity study, sputum for quantitative culture and mRNA analysis was collected for 2 days before and daily during 7 days of study drug administration. In the IL-2 trial, sputum was collected for quantitative culture, Bactec 460 liquid culture, and mRNA analysis throughout the intensive treatment phase. RNA was isolated from digested sputum and tested in quantitative reverse transcription-PCR assays for several gene targets. mRNA for the glyoxylate cycle enzyme isocitrate lyase declined at similar rates in patients receiving isoniazid, gatifloxicin, levofloxacin, and moxifloxacin monotherapy. Isocitrate lyase mRNA correlated highly with CFU in sputum prior to therapy and during 7 days of monotherapy in all treatment arms. Isocitrate lyase mRNA was detectable in sputum of culture-positive TB patients receiving a rifampin-based regimen for 1 month. At 2 months, sputum for isocitrate mRNA correlated more closely with growth in liquid culture than did growth on solid culture medium. Data suggest that isocitrate lyase mRNA is a reliable marker of M. tuberculosis viability. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Li, L.
United States, Little Rock
University of Arkansas for Medical Sciences
Mahan, C. Scott
United States, Cleveland
Metrohealth Medical Center Cleveland
United States, Cleveland
University Hospitals Case Medical Center
Palaci, Moises
Brazil, Vitoria
Universidade Federal do Espírito Santo
Horter, Libby
United States, Cleveland
University Hospitals Case Medical Center
Loeffelholz, L.
United States, Bloomington
Normandale Community College
Johnson, John L.
United States, Cleveland
University Hospitals Case Medical Center
Dietze, Reynaldo
Brazil, Vitoria
Universidade Federal do Espírito Santo
Debanne, Sara M.
United States, Cleveland
Case School of Medicine
Joloba, Moses Lutaakome
Uganda, Kampala
Joint Clinical Research Center Uganda
Uganda, Kampala
School of Medicine, Makerere University College of Health Sciences
Okwera, Alphonse
Uganda, Kampala
Natl. tb and Leprosy Contr. Prog.
Boom, W. Henry
United States, Cleveland
University Hospitals Case Medical Center
Eisenach, Kathleen D.
United States, Little Rock
University of Arkansas for Medical Sciences
Statistics
Citations: 61
Authors: 12
Affiliations: 9
Identifiers
Doi:
10.1128/JCM.01526-09
ISSN:
00951137
Research Areas
Genetics And Genomics
Infectious Diseases
Study Approach
Quantitative