Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Reactive Oxygen Species Production by Monocytes and Polymorphonuclear Leukocytes During Dialysis
American Journal of Kidney Diseases, Volume 17, No. 3, Year 1991
Notification
URL copied to clipboard!
Description
Intradialytic production of reactive oxygen species (ROS) by monocytes and polymorphonuclear leukocytes (PMNL) was examined separately in six hemodialysis patients. Samples obtained 15 minutes after initiation of dialysis with new cuprophane membanes demonstrated significantly increased (P < 0.05) ROS production in both cell populations as measured by the fluorescence of a specific intracellular marker (2′,7′-dichlorofluorescein diacetate [DCFH-DA]) assayed by flow cytometry. Granulocytes harvested during dialysis also showed decreased responsiveness to exogenous C5a and F-Met-Leu-Phe (FMLP) at 15, 30, and 60 minutes after initiation of dialysis (P < 0.05). Our data suggest that hemodialysis with cuprophane membrane is associated with monocyte and PMNL activation as shown by production of ROS coincident with peak activation of the complement cascade; these granulocytes become refractory to further stimulation with C5a and FMLP during dialysis. © 1991, National Kidney Foundation, Inc.. All rights reserved.
Authors & Co-Authors
Lazarus, John Michael
United States, Portland
Maine Medical Center
United States, Boston
Harvard Medical School
United States, Nashville
Vanderbilt University Medical Center
Statistics
Citations: 176
Authors: 1
Affiliations: 3
Identifiers
Doi:
10.1016/S0272-6386(12)80473-2
ISSN:
02726386
Research Areas
Noncommunicable Diseases