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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Importance of both the coding and the segment-specific noncoding regions of the influenza A virus NS segment for its efficient incorporation into virions
Journal of Virology, Volume 79, No. 6, Year 2005
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Description
The genome of influenza A virus consists of eight single-strand negative-sense RNA segments, each comprised of a coding region and a noncoding region. The noncoding region of the NS segment is thought to provide the signal for packaging; however, we recently showed that the coding regions located at both ends of the hemagglutinin and neuraminidaise segments were important for their incorporation into virions. In an effort to improve our understanding of the mechanism of influenza virus genome packaging, we sought to identify the regions of NS viral RNA (vRNA) that are required for its efficient incorporation into virions. Deletion analysis showed that the first 30 nucleotides of the 3′ coding region are critical for efficient NS vRNA incorporation and that deletion of the 3′ segment-specific noncoding region drastically reduces NS vRNA incorporation into virions. Furthermore, silent mutations in the first 30 nucleotides of the 3′ NS coding region reduced the incorporation efficiency of the NS segment and affected virus replication. These results suggested that segment-specific noncoding regions together with adjacent coding regions (especially at the 3′ end) form a structure that is required for efficient influenza A virus vRNA packaging. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Noda, Takeshi
Japan, Sapporo
Hokkaido University
Watanabe, Tokiko
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Takada, Ayato
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Goto, Hideo
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Horimoto, Taisuke
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Kawaoka, Yoshihiro K.
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
United States, Madison
University of Wisconsin-madison
Statistics
Citations: 160
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1128/JVI.79.6.3766-3774.2005
ISSN:
0022538X