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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Impedance biosensing using phages for bacteria detection: Generation of dual signals as the clue for in-chip assay confirmation
Biosensors and Bioelectronics, Volume 26, No. 4, Year 2010
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Description
In the present work, we compare the use of antibodies (Ab) and phages as bioreceptors for bacteria biosensing by Electrochemical Impedance Spectroscopy (EIS). With this aim, both biocomponents have been immobilised in parallel onto interdigitated gold microelectrodes. The produced surfaces have been characterised by EIS and Fourier Transform Infra-Red (FTIR) Spectroscopy and have been applied to bacteria detection. Compared to immunocapture, detection using phages generates successive dual signals of opposite trend over time, which consist of an initial increase in impedance caused by bacteria capture followed by impedance decrease attributed to phage-induced lysis. Such dual signals can be easily distinguished from those caused by non-specific adsorption and/or crossbinding, which helps to circumvent one of the main drawbacks of reagentless biosensors based in a single target-binding event. The described strategy has generated specific detection of Escherichia coli in the range of 104-107CFUmL-1 and minimal interference by non-target Lactobacillus. We propose that the utilisation of phages as capture biocomponent for bacteria capture and EIS detection allows in-chip signal confirmation. © 2010 Elsevier B.V.
Authors & Co-Authors
Mejri, M. B.
Tunisia, Tunis
University of Carthage, Institut National Des Sciences Appliquées et de Technologie
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Baccar, Hamdi
Tunisia, Tunis
University of Carthage, Institut National Des Sciences Appliquées et de Technologie
Baldrich, Eva
Spain, Cerdanyola Del Valles
Csic - Instituto de Microelectronica de Barcelona Imb-cnm
del Campo, Francisco Javier
Spain, Cerdanyola Del Valles
Csic - Instituto de Microelectronica de Barcelona Imb-cnm
Helali, Saloua
Tunisia, Tunis
University of Carthage, Institut National Des Sciences Appliquées et de Technologie
Ktari, Taha
Tunisia, Tunis
University of Carthage, Institut National Des Sciences Appliquées et de Technologie
Simonian, A.
United States, Auburn
Auburn University
Aouni, Mahjoub
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Abdelghani, Adnane
Tunisia, Tunis
University of Carthage, Institut National Des Sciences Appliquées et de Technologie
Statistics
Citations: 82
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1016/j.bios.2010.06.054
ISSN:
09565663