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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
High-performance liquid chromatographic method for determination of amodiaquine, chloroquine and their monodesethyl metabolites in biological samples
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Volume 783, No. 2, Year 2003
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Description
A high-performance liquid chromatographic method for determination of amodiaquine (AQ), desethylamodiaquine (DAQ), chloroquine (CQ) and desethylchloroquine (DCQ) in human whole blood, plasma and urine is reported. 4-(4-Dimethylamino-1-methylbutylamino)-7-chloroquinoline was used as internal standard. The drugs and the internal standard were extracted into di-isopropyl ether as bases and then re-extracted into an acidic aqueous phase with 0.1 M phosphate buffer at pH 4.0 for AQ samples and at pH 2.5 for CQ filter paper samples. A C18 column was used and the mobile phase consisted of methanol-phosphate buffer (0.1 M, pH 3)-perchloric acid (250: 747.5:2.5, v/v). The absorbance of the drugs was monitored at 333 nm and no endogenous compound interfered at this wavelength. The limit of quantification in whole blood, plasma and urine was 100 nM for AQ and DAQ (sample size 100 μl) as well as for CQ and DCQ in blood samples dried on filter paper. For 1000 μl AQ and DAQ samples, the limit of quantification was 10 nM in all three biological fluids. The within-assay and between-assay coefficients of variations were always <10% at the limits of quantification. Plasma should be preferred for the determination of AQ and DAQ since use of whole blood may be associated with stability problems. © 2002 Elsevier Science B.V. All rights reserved.
Authors & Co-Authors
Minzi, Omary Mashiku Sylivester
Tanzania, Dar es Salaam
Muhimbili University of Health and Allied Sciences
Rais, Margareta
Sweden, Stockholm
Karolinska Institutet
Svensson, Jan Olof
Sweden, Stockholm
Karolinska Universitetssjukhuset
Gustafsson, Lars L.
Sweden, Stockholm
Karolinska Institutet
Ericsson, Örjan T.
Sweden, Stockholm
Karolinska Institutet
Statistics
Citations: 61
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/S1570-0232(02)00727-4
ISSN:
15700232