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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Diagnosis of Trichomonous vaginalis by microscopy, latex agglutination, diamond's media, and PCR in symptomatic women, Khartoum, Sudan
Diagnostic Pathology, Volume 9, No. 1, Article 49, Year 2014
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Description
Background: Trichomoniasis is the most common sexually transmitted disease. However, limited data are available on an effective technique for the diagnosis of Trichomonas vaginalis.Methods: A cross-sectional study was conducted to evaluate the accuracy of wet mount microscopy, latex agglutination, Diamond's media, and polymerase chain reaction (PCR) for detection of T. vaginalis among symptomatic women who attended the gynecological clinic at Khartoum, Sudan.Results: Of the 297 women studied, 252 (84.8%) were positive for T. vaginalis by wet mount microscopy, 257 (86.5%) by latex agglutination, 253 (85.2%) by Diamond's media, and 253 (85.2%) by PCR. The sensitivity and specificity of wet mount microscopy were 99.2% and 97.7%, respectively, compared with PCR. The sensitivity and specificity of latex agglutination and Diamond's media were 99.6% and 88.6%, and 100.0% and 86.4%, respectively, compared with PCR.Conclusions: In this study, wet mount microscopy, latex agglutination, and Diamond's media were found to be highly sensitive and specific. However, the availability and cost effectiveness might limit the use of Diamond's media and PCR in routine practice. © 2014 Saleh et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Saleh, Amir M.
Sudan
University of Omdurman
Abdalla, Hamid Sulaiman
Sudan
University of Omdurman
Satti, Abdelsalam
Sudan
University of Omdurman
Babiker, Suad M.
Sudan
University of Omdurman
Gasim, Gasim I.
Saudi Arabia, Buraidah
Al Qassim University
Adam, Ishag I.
Sudan, Khartoum
University of Khartoum Faculty of Medicine
Statistics
Citations: 18
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1186/1746-1596-9-49
e-ISSN:
17461596
Research Areas
Health System And Policy
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Sudan
Participants Gender
Female