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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Biological characterization and complete nucleotide sequence of a Tunisian isolate of Moroccan watermelon mosaic virus
Archives of Virology, Volume 153, No. 1, Year 2008
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Description
During a survey conducted in October 2005, cucurbit leaf samples showing virus-like symptoms were collected from the major cucurbit-growing areas in Tunisia. DAS-ELISA showed the presence of Moroccan watermelon mosaic virus (MWMV, Potyvirus), detected for the first time in Tunisia, in samples from the region of Cap Bon (Northern Tunisia). MWMV isolate TN05-76 (MWMV-Tn) was characterized biologically and its full-length genome sequence was established. MWMV-Tn was found to have biological properties similar to those reported for the MWMV type strain from Morocco. Phylogenetic analysis including the comparison of complete amino-acid sequences of 42 potyviruses confirmed that MWMV-Tn is related (65% amino-acid sequence identity) to Papaya ringspot virus (PRSV) isolates but is a member of a distinct virus species. Sequence analysis on parts of the CP gene of MWMV isolates from different geographical origins revealed some geographic structure of MWMV variability, with three different clusters: one cluster including isolates from the Mediterranean region, a second including isolates from western and central Africa, and a third one including isolates from the southern part of Africa. A significant correlation was observed between geographic and genetic distances between isolates. Isolates from countries in the Mediterranean region where MWMV has recently emerged (France, Spain, Portugal) have highly conserved sequences, suggesting that they may have a common and recent origin. MWMV from Sudan, a highly divergent variant, may be considered an evolutionary intermediate between MWMV and PRSV. © 2007 Springer-Verlag.
Authors & Co-Authors
Yakoubi, Soumaya
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Desbiez, Cécile
France, Avignon
Pathologie Végétale pv
Fakhfakh, Hatem
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Wipf-Scheibel, Catherine
France, Avignon
Pathologie Végétale pv
Marrakchi, Mohammed
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Lecoq, Hervé
France, Avignon
Pathologie Végétale pv
Statistics
Citations: 31
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1007/s00705-007-1074-2
ISSN:
03048608
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Morocco
Sudan
Tunisia