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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A549 lung cell line activity of biosynthesized silver nanoparticles using Albizia adianthifolia leaf
Colloids and Surfaces B: Biointerfaces, Volume 105, Year 2013
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Description
Stable AgNPs were formed in vitro by reacting AgNO3 (aq) solution with the aqueous plant leaf extract. UV-vis revealed the surface plasmon resonance λmax at 448nm and the absorbance steadily increased in intensity as a function of reaction time. Transmission electron microscope (TEM) and XRD studies were used to characterize the AgNPs; the size was 4-35nm. Dynamic light scattering (DLS) was used as supporting evidence to determine hydrodynamic size and zeta potential recorded as 80.27nm and -24.7mV, respectively. FT-IR spectra suggest that AgNPs are capped with protein molecules and other water soluble phytocompounds such as saponins and glycosides which also behave as stabilizing agents; TEM images indicate a visible layer surrounding the AgNPs. Prominent absorption bands at 3380 and 1642cm-1 are assigned to alcohol and carbonyl groups, respectively. 1H NMR of the neat aqueous plant extract indicates presence of a complex mixture of compounds; however the chemical shift at δ 6.0-8.0 and 1.0-4.0ppm indicates the presence of few aromatic but abundant aliphatic compounds, respectively. Toxicity of AgNPs on lung cancer cells (A549) and normal healthy peripheral lymphocytes (PLs) at 10. μg/ml and 50. μg/ml was assessed using the MTT, ATP and lactate dehydrogenase assays. Viability data for A549 cells showed a 21% (10. μg/ml) and 73% (50. μg/ml) cell viability after 6. h exposure to AgNPs compared to 117% (10. μg/ml) and 109% (50. μg/ml) cell viability of normal peripheral lymphocytes. Lactate dehydrogenase was only significantly altered at 50. μg/ml AgNPs treated cells from 2.43. ±0.04 units to 0.77. ±0.04 units. © 2013 Elsevier B.V.
Authors & Co-Authors
Gengan, Robert Moonsamy
South Africa, Durban
Durban University of Technology
Anand, Krishnan
South Africa, Durban
Durban University of Technology
Phulukdaree, Alisa
South Africa, Durban
The Nelson R. Mandela Medical School
Chuturgoon, Anil Amichund
South Africa, Durban
The Nelson R. Mandela Medical School
Statistics
Citations: 178
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/j.colsurfb.2012.12.044
ISSN:
09277765
e-ISSN:
18734367
Research Areas
Cancer
Environmental
Substance Abuse