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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Size determination of bacterial capsular oligosaccharides used to prepare conjugate vaccines against Neisseria meningitidis groups Y and W135
Vaccine, Volume 23, No. 16, Year 2005
Notification
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Description
The glycoconjugate vaccines against Neisseria meningitidis groups Y and W135 consist of pools of selected oligosaccharides conjugated to the protein carrier (CRM197). Consistent production of these vaccines requires control and thus determination of the average degree of polymerisation of the oligosaccharides used for conjugation. Acid hydrolysis generates group Y and W135 oligosaccharides with N-acetylneuraminic acid at the reducing end. A method, involving NaBH4 reduction and quantification of this terminal N-acetylneuraminic acid by use of high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) following acid hydrolysis (2 M TFA), was developed. The average degree of polymerisation is calculated from the ratio of reduced N-acetylneuraminic acid to total N-acetylneuraminic acid. The assay was qualified by application to group C, Y and W135 oligosaccharide standards characterised by liquid chromatography, mass and NMR spectroscopy. © 2004 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Bardotti, Angela
Italy, Siena
Novartis Immunobiological Research Institute
Averani, Giovanni
Italy, Siena
Novartis Immunobiological Research Institute
Berti, Francesco
Italy, Siena
Novartis Immunobiological Research Institute
Berti, Stefania
Italy, Siena
Novartis Immunobiological Research Institute
Galli, Chiara
Italy, Siena
Novartis Immunobiological Research Institute
Giannini, Sara
Italy, Siena
Novartis Immunobiological Research Institute
Fabbri, Barbara
Italy, Siena
Novartis Immunobiological Research Institute
Proietti, Daniela
Italy, Siena
Novartis Immunobiological Research Institute
Ravenscroft, Neil
South Africa, Cape Town
University of Cape Town
Ricci, Stefano
Italy, Siena
Novartis Immunobiological Research Institute
Statistics
Citations: 10
Authors: 10
Affiliations: 2
Identifiers
Doi:
10.1016/j.vaccine.2004.10.021
ISSN:
0264410X