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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Inhibition of the secretory pathway by foot-and-mouth disease virus 2BC protein is reproduced by coexpression of 2B with 2C, and the site of inhibition is determined by the subcellular location of 2C
Journal of Virology, Volume 81, No. 3, Year 2007
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Description
Infection of cells with picornaviruses can lead to a block in protein secretion. For poliovirus this is achieved by the 3A protein, and the consequent reduction in secretion of proinflammatory cytokines and surface expression of major histocompatibility complex class I proteins may inhibit host immune responses in vivo. Foot-and-mouth disease virus (FMDV), another picornavirus, can cause persistent infection of ruminants, suggesting it too may inhibit immune responses. Endoplasmic reticulum (ER)-to-Golgi apparatus transport of proteins is blocked by the FMDV 2BC protein. The observation that 2BC is processed to 2B and 2C during infection and that individual 2B and 2C proteins are unable to block secretion stimulated us to study the elects of 2BC processing on the secretory pathway. Even though 2BC was processed rapidly to 2B and 2C, protein transport to the plasma membrane was still blocked in FMDV-infected cells. The block could be reconstituted by coexpression of 2B and 2C, showing that processing of 2BC did not compromise the ability of FMDV to slow secretion. Under these conditions, 2C was located to the Golgi apparatus, and the block in transport also occurred in the Golgi apparatus. Interestingly, the block in transport could be redirected to the ER when 2B was coexpressed with a 2C protein fused to an ER retention element. Thus, for FMDV a block in secretion is dependent on both 2B and 2C, with the latter determining the site of the block. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Moffat, Katy
United Kingdom, Surrey
The Pirbright Institute
Knox, Caroline M.
United Kingdom, St Andrews
University of st Andrews
South Africa, Grahamstown
Rhodes University
Howell, Gareth
United Kingdom, Surrey
The Pirbright Institute
United Kingdom, Leeds
University of Leeds
Clark, Sarah J.
United Kingdom, Surrey
The Pirbright Institute
Yang, H.
United Kingdom, Surrey
The Pirbright Institute
Belsham, G.
United Kingdom, Surrey
The Pirbright Institute
Denmark, Lyngby
Technical University of Denmark
Ryan, Martin
United Kingdom, St Andrews
University of st Andrews
Wileman, Thomas
United Kingdom, Surrey
The Pirbright Institute
United Kingdom, Norwich
University of East Anglia, Norwich Medical School
Statistics
Citations: 106
Authors: 8
Affiliations: 6
Identifiers
Doi:
10.1128/JVI.00393-06
ISSN:
0022538X