Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Dynamics of the Toxoplasma gondii inner membrane complex
Journal of Cell Science, Volume 127, No. 15, Year 2014
Notification
URL copied to clipboard!
Description
Unlike most cells, protozoa in the phylum Apicomplexa divide by a distinctive process in which multiple daughters are assembled within the mother (schizogony or endodyogeny), using scaffolding known as the inner membrane complex (IMC). The IMC underlies the plasma membrane during interphase, but new daughters develop in the cytoplasm, as cytoskeletal filaments associate with flattened membrane cisternae (alveolae), which elongate rapidly to encapsulate subcellular organelles. Newly assembled daughters acquire their plasma membrane as they emerge from the mother, leaving behind vestiges of the maternal cell. Although the maternal plasma membrane remains intact throughout this process, the maternal IMC disappears -is it degraded, or recycled to form the daughter IMC? Exploiting fluorescently tagged IMC markers, we have used live-cell imaging, fluorescence recovery after photobleaching (FRAP) and mEos2 photoactivation to monitor the dynamics of IMC biogenesis and turnover during the replication of Toxoplasma gondii tachyzoites. These studies reveal that the formation of the T. gondii IMC involves two distinct steps - de novo assembly during daughter IMC elongation within the mother cell, followed by recycling of maternal IMC membranes after the emergence of daughters from the mother cell. © 2014. Published by The Company of Biologists Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4134349/bin/supp_127_15_3320__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC4134349/bin/supp_127.15.3320_JCS147736.pdf
Authors & Co-Authors
Ouologuem, Dinkorma T.
United States, Philadelphia
University of Pennsylvania
Mali, Bamako
Malaria Research and Training Centre
Roos, David S.
United States, Philadelphia
University of Pennsylvania
Statistics
Citations: 51
Authors: 2
Affiliations: 2
Identifiers
Doi:
10.1242/jcs.147736
ISSN:
00219533
e-ISSN:
14779137
Research Areas
Maternal And Child Health