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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Isolation and molecular characterization of Staphylococcus sciuri in the hospital environment
Journal of Clinical Microbiology, Volume 43, No. 6, Year 2005
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Description
Staphylococcus sciuri is a principally animal-associated bacterial species, but its clinical relevance for humans is increasing. Our study aimed to provide the first insight into the prevalence of this bacterium in a hospital environment. A 3-month surveillance was conducted in a hospital located in Belgrade, Serbia, and 1,028 samples taken from hands of medical personnel, medical devices, and various hospital surfaces were screened for S. sciuri presence. In total, 108 isolates were obtained, which resulted in a relatively high rate of colonization (10.5%). These isolates, along with 7 S. sciuri strains previously isolated in the same hospital (n = 115), were phenotypically and genotypically characterized. Antimicrobial susceptibility testing revealed that 73% of the strains were resistant to one or more antibiotics, with 4.3% strains displaying multiresistance. Examination of 16S-23S ribosomal DNA intergenic spacer length polymorphism identified the strains at the subspecies level, and 74 (64.3%) strains of S. sciuri subsp. sciuri, 37 (32.2%) strains of S. sciuri subsp. rodentium, and 4 (3.5%) strains of S. sciuri subsp. carnaticus were established. Pulsed-field gel electrophoresis (PFGE) analysis showed 21 distinct pulsotypes, including 17 main types and 4 subtypes. One dominant cluster with 62 strains was found, while 19 (90.5%) of the PFGE types and subtypes identified had 5 or fewer strains. The predominance of small PFGE clusters suggests that the ubiquitous presence of S. sciuri in the outside environment presents the continuous source for colonization of the hospital environment. The presence of one dominant PFGE cluster of strains indicates that some S. sciuri strains may be capable for adaptation to hospital environment conditions and continuous existence in this environment. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Dakić, Ivana R.
Serbia, Belgrade
Belgrade University School of Medicine
Morrison, Donald
United Kingdom, Glasgow
New Stobhill Hospital
Vukovic, Dragana
Serbia, Belgrade
Belgrade University School of Medicine
Savić, Branislava
Serbia, Belgrade
Belgrade University School of Medicine
Shittu, Adebayo
South Africa, Durban
University of Kwazulu-natal
Ježek, Petr
Serbia, Pribram
Regional Hospital Příbram
Hauschild, Tomasz
Poland, Bialystok
Uniwersytet w Bialymstoku
Stepanović, Srdjan
Serbia, Belgrade
Belgrade University School of Medicine
Statistics
Citations: 8
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1128/JCM.43.6.2782-2785.2005
ISSN:
00951137
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study