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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis
Acta Tropica, Volume 102, No. 3, Year 2007
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Description
In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to ∼0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that lack sufficient resources needed for application of molecular diagnostic techniques. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Thekisoe, Oriel M.M.
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Kuboki, Noritaka
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Nambota, Andrew Mubila
Zambia, Lusaka
University of Zambia
Fujisaki, Kozo
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Sugimoto, Chihiro
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Igarashi, Ikuo
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Yasuda, Jun
Japan, Morioka
Iwate University
Inoue, Noboru I.
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Statistics
Citations: 142
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1016/j.actatropica.2007.05.004
ISSN:
0001706X
Research Areas
Genetics And Genomics