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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Distinct phenotype of SARS-CoV-2 Omicron BA.1 in human primary cells but no increased host range in cell lines of putative mammalian reservoir species
Virus Research, Volume 339, Article 199255, Year 2024
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Description
SARS-CoV-2′s genetic plasticity has led to several variants of concern (VOCs). Here we studied replicative capacity for seven SARS-CoV-2 isolates (B.1, Alpha, Beta, Gamma, Delta, Zeta, and Omicron BA.1) in primary reconstituted airway epithelia (HAE) and lung-derived cell lines. Furthermore, to investigate the host range of Delta and Omicron compared to ancestral SARS-CoV-2, we assessed replication in 17 cell lines from 11 non-primate mammalian species, including bats, rodents, insectivores and carnivores. Only Omicron's phenotype differed in vitro, with rapid but short replication and efficient production of infectious virus in nasal HAEs, in contrast to other VOCs, but not in lung cell lines. No increased infection efficiency for other species was observed, but Delta and Omicron infection efficiency was increased in A549 cells. Notably replication in A549 and Calu3 cells was lower than in nasal HAE. Our results suggest better adaptation of VOCs towards humans, without an extended host range, and may be relevant to the search for the putative intermediate host and reservoirs prior to the pandemic. © 2023
Authors & Co-Authors
Pérez-Rodriguez, Francisco Javier
Switzerland, Geneva
Université de Genève
Switzerland, Geneva
Hôpitaux Universitaires de Genève
Bekliz, Meriem
Switzerland, Geneva
Université de Genève
Lenk, Matthias
Germany, Tubingen
Friedrich-loeffler-institute
Suliman, Tasnim
South Africa, Bellville
University of the Western Cape
Preiser, Wolfgang
South Africa, Stellenbosch
Stellenbosch University
South Africa, Johannesburg
National Health Laboratory Service
Müller, Marcel A.
Germany, Berlin
Charité – Universitätsmedizin Berlin
Kaiser, Laurent K.
Switzerland, Geneva
Université de Genève
Switzerland, Geneva
Hôpitaux Universitaires de Genève
Eckerle, Isabella Anne
Switzerland, Geneva
Université de Genève
Switzerland, Geneva
Hôpitaux Universitaires de Genève
Statistics
Authors: 8
Affiliations: 7
Identifiers
Doi:
10.1016/j.virusres.2023.199255
ISSN:
01681702
Research Areas
Genetics And Genomics