Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
SLC26A4 expression among autoimmune thyroid tissues
Immunobiology, Volume 216, No. 5, Year 2011
Notification
URL copied to clipboard!
Description
Context: The PDS gene (SLC26A4) is responsible for Pendred syndrome (PS). Genetic analysis of PDS using Tunisian samples showed evidence for linkage and association with autoimmune thyroid diseases (AITD) emergence. In addition, the PDS gene product, pendrin, was recently identified as a novel autoantigen in Graves' disease (GD) or Hashimoto thyroiditis (HT) patients' sera. Objective: The aim of this study was to quantify the PDS gene expression and to evaluate the pendrin in vivo and in vitro immunolocalisation. Patients: A total of 52 thyroid gland tissue samples (22 GD, 11 HT, 5 multinodular goiter (MNG), 3 normal thyroid tissues, 8 papillary thyroid carcinoma (PTC), 1 follicular thyroid carcinoma (FTC) and 2 medullar thyroid carcinoma (MTC)) were explored.Method PDS and pendrin expression levels were determined using quantitative RT-PCR and immuno-detection methods. TSH and thyroglobulin (Tg) effects on pendrin expression were investigated by immunofluorescence on primary cell culture from GD thyroid tissues. Results: The relative quantification using PDS transcript level among GD thyroid tissues was increased compared to normal thyroid tissues used as calibrator (mean: 27.17-fold higher than normal thyroid tissues). However, thyroids with HT, carcinoma and MNG showed a decrease expression level (means: 92.05-, 77.68-, 14.3-fold lower than normal thyroid tissues, respectively). These results were confirmed by immunoanalysis. Immunofluorescence results showed an apical and a cytoplasmic pendrin localisation on GD thyroid tissues and a marked pendrin expression reduction on HT thyroid tissues. GD primary cell cultures under TSH and Tg stimulation showed a trafficking improvement of pendrin apical localisation. Conclusions: Our data point to the presence of a relation between SLC26A4 expression in AITD and thyroid function. © 2010 Elsevier GmbH.
Authors & Co-Authors
Belguith-Maalej, Salima
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Tunisia
Laboratoire International Associé Lia-135
Rebuffat, Sandra
France, Paris
Cnrs Centre National de la Recherche Scientifique
Tunisia
Laboratoire International Associé Lia-135
Charfeddine, Ilhem
Tunisia, Sfax
Chu Habib Bourguiba
Mnif, Mouna Feki
Tunisia, Sfax
Chu Habib Bourguiba
Tunisia
Laboratoire International Associé Lia-135
Nadir, R. Farid
United Kingdom, London
London Endocrine Clinic
Abid, Mohamed Salah
Tunisia, Sfax
Chu Habib Bourguiba
Ghorbel, Abdelmonem
Tunisia, Sfax
Chu Habib Bourguiba
Péraldi-Roux, Sylvie
France, Paris
Cnrs Centre National de la Recherche Scientifique
Tunisia
Laboratoire International Associé Lia-135
Ayadi, Hammadi
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Tunisia
Laboratoire International Associé Lia-135
Hadj Kacem, Hassen
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Tunisia
Laboratoire International Associé Lia-135
Statistics
Citations: 10
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1016/j.imbio.2010.09.015
ISSN:
01712985
Research Areas
Cancer
Genetics And Genomics
Study Approach
Quantitative