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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1
Clinica Chimica Acta, Volume 354, No. 1-2, Year 2005
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Description
Objective: As alkaline phosphatase may play a role in cell differentiation, our aim was to study the possible role of this enzyme in the differentiation of preadipocytes (3T3-L1 cells) into adipocytes. Research Methods and Procedures: 3T3-L1 cells were grown in medium containing insulin, dexamethasone and IBMX to induce adipogenesis. Adipogenesis was measured using the triglyceride-specific dye, oil red O at 0, 3, 7 and 11 days after initiation of adipogenesis in the presence or absence of the alkaline phosphatase inhibitors, levamisole, histidine and Phe-Gly-Gly. Intracellular localisation of the enzyme was detected using ELF-phosphatase, a fluorescent substrate and alkaline phosphatase gene expression was assessed using RT-PCR. Results: Alkaline phosphatase activity was detected in untransformed cells (1.91±0.62 mU/mg protein) and activity increased 11.5±1.4-fold after 11 days treatment with transformation medium and 5.3±0.3-fold in transformation medium containing levamisole (p<0.05). Triglyceride content of cells increased 3.1±0.2-fold after 11 days treatment with transformation medium and 2.1±0.3-fold in the presence of levamisole (p<0.005). Histidine inhibited adipogenesis and alkaline phosphatase to a greater extent than did levamisole, but Phe-Gly-Gly had no effect on these variables. Alkaline phosphatase was localised around the lipid droplets of the cells. Gene expression of alkaline phosphatase increased during adipogenesis. Discussion: This study demonstrates that tissue-nonspecific alkaline phosphatase is present in 3T3-L1 cells and that it may play a role in the control of adipogenesis. © 2004 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Ali, Aus Tariq
South Africa, Johannesburg
University of the Witwatersrand Faculty of Health Sciences
Penny, Clement B.
South Africa, Johannesburg
University of the Witwatersrand Faculty of Health Sciences
Paiker, Janice E.
South Africa, Johannesburg
University of the Witwatersrand Faculty of Health Sciences
van Niekerk, Chantal
South Africa, Johannesburg
University of the Witwatersrand Faculty of Health Sciences
Smit, Aletta
South Africa, Johannesburg
University of the Witwatersrand Faculty of Health Sciences
Ferris, William Frank
South Africa, Stellenbosch
Stellenbosch University
Crowther, Nigel John
South Africa, Johannesburg
University of the Witwatersrand Faculty of Health Sciences
Statistics
Citations: 81
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.cccn.2004.11.026
ISSN:
00098981
Research Areas
Genetics And Genomics