Identification, cloning, expression of a novel functional anasplatyrhynchos mRNA TLR4

Toll like receptor mediate immune response through recognition of Pathogen-Associated Molecular Patterns (PAMPs) thus play important roles in host defense. Little is known about the Avian Immune System structure and the majority of it based on chicken as a Universal Avian Model while recent researches showed that a lot of factor not common between chicken and other birds. This research aimed to identify and clone the duck TLR4 as a model to Anseriformes species, researchers success to clone a novel functional dTLR4 mRNA and deposit into the NCBI GenBank database where the mRNA consist of 2529 nucleotide and it showed 81 % identity with chicken and 78% identity with zebra finch while showed 95% identity with goose. The translated amino acid showed that the gene consist of 843 amino acid and the transmembrane structure analysis reveal a novel unique LRR motifs in duck and absent in both chicken and zebra finch at the position 56-80 also showed that TIR domain consist of 146 amino acid not 145 like chicken and zebra finch. © 2012 Medwell Journals.