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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Development of thrombus-resistant and cell compatible crimped polyethylene terephthalate cardiovascular grafts using surface co-immobilized heparin and collagen
Materials Science and Engineering C, Volume 43, Year 2014
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Description
Short-term patency of polyethylene terephthalate (PET) cardiovascular grafts is determined mainly by the inherent thrombogenicity and improper endothelialization following grafts implantation. The aim of the present study was to immobilize heparin to develop thrombus resistant grafts. Additionally, collagen was co-immobilized to enhance the host cell compatibility. The synthetic woven and knitted forms of crimped PET grafts were surface modified by Denier reduction to produce functional carboxyl groups. The produced groups were used as anchor sites for covalent immobilization of heparin or co-immobilization of heparin/collagen by the end-point method. The modified surface was characterized using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The biological activity of immobilized molecules was investigated in vitro using direct blood coagulation test, and "platelet deposition under flow condition. Furthermore, the biocompatibility of modified grafts with host cells was assessed using L929 cell as model. All modified grafts showed significant resistance against fibrin and clot formation. The number of deposited platelets on heparin-immobilized woven and knitted grafts obviously decreased by 3 fold and 2.8 fold per unit surface area respectively, while the heparin/collagen co-immobilized grafts showed only a decrease by 1.7 and 1.8 fold compared to unmodified PET. Heparin-immobilized grafts reported no significant effect on L929 cells adhesion and growth (P > 0.05), conversely, collagen co-immobilization considerably increased cell adhesion almost ~ 1.3 fold and 2 fold per unit surface area for woven and knitted grafts respectively. Our results emphasize that immobilization of heparin minimized the inherent thrombogenicity of the PET grafts. The simultaneous co-immobilization of collagen supported host cell adhesion and growth required for the grafts biocompatibility. © 2014 Elsevier B.V.
Authors & Co-Authors
Al Meslmani, Bassam
Germany, Marburg
Philipps-universität Marburg
Mahmoud, Gihan
Germany, Marburg
Philipps-universität Marburg
Egypt, Helwan
Helwan University
Strehlow, Boris
Germany, Marburg
Philipps-universität Marburg
Mohr, Eva
Germany, Marburg
Philipps-universität Marburg
Leichtweiß, Thomas
Germany, Giessen
Justus-liebig-universität Gießen
Bakowsky, Udo
Germany, Marburg
Philipps-universität Marburg
Statistics
Citations: 38
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.msec.2014.07.059
ISSN:
09284931
Research Areas
Noncommunicable Diseases