Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Use of FTA cards for direct sampling of patients' lesions in the ecological study of cutaneous leishmaniasis
Journal of Clinical Microbiology, Volume 48, No. 10, Year 2010
Notification
URL copied to clipboard!
Description
The FTA card (Whatman) was assessed for its utility as a molecular epidemiological tool in collecting samples from patients with leishmaniasis in Peru because the card has a variety of merits; it is less invasive for patients and easy to handle for both physicians and other medical personnel for sample collection or diagnosis, in addition to its simplicity and easy countrywide and/or intercountry transportation for analysis. Samples were collected from 132 patients suspected of having leishmaniasis, and Leishmania species were successfully identified in samples from 81 patients in 15 departments of Peru by cytochrome b and mannose phosphate isomerase gene analyses. Of these, 61.7% were identified as Leishmania (Viannia) peruviana, 22.2% as L. (V.) braziliensis, 12.3% as L. (V.) guyanensis, 2.5% as L. (V.) shawi, and 1.2% as L. (V.) lainsoni. The three predominant species, L. (V.) peruviana, L. (V.) braziliensis, and L. (V.) guyanensis, were mainly found in the Andean highlands, in the tropical rainforest, and in northern and central rainforest regions, respectively. This is the first time L. (V.) shawi has been identified outside Brazil. The present study showed that the FTA card will be a useful tool for the ecological study of different forms of leishmaniasis. Furthermore, collecting samples directly from patients' lesions by using the FTA card eliminates (i) the possibility of contamination of Leishmania isolates during short- and/or long-term passages of culture in vitro in each laboratory and (ii) pain and suffering of patients from taking samples by skin biopsy. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Kato, Hirotomo
Japan, Yamaguchi
Yamaguchi University
Cáceres, Abraham G.
Peru, Lima
Instituto de Medicina Tropical Daniel A. Carrión
Peru, Lima
Instituto Nacional de Salud, Lima
Mimori, Tatsuyuki
Japan, Kumamoto
Kumamoto University School of Health Sciences
Ishimaru, Yuka
Japan, Yamaguchi
Yamaguchi University
Sayed, Amal S.M.
Japan, Yamaguchi
Yamaguchi University
Egypt, Asyut
Faculty of Veterinary Medicine
Fujita, Megumi
Japan, Yamaguchi
Yamaguchi University
Iwata, Hiroyuki
Japan, Yamaguchi
Yamaguchi University
Uezato, Hiroshi
Japan, Nishihara
University of the Ryukyus
Velez, Lenin N.
Ecuador
Departamento de Leishmaniasis
Gomez, Eduardo A.L.
Ecuador, Guayaquil
Servicio Nacional de Erradicacion de la Malaria Snem
Hashiguchi, Y.
Japan, Kochi
Kochi University
Statistics
Citations: 63
Authors: 11
Affiliations: 9
Identifiers
Doi:
10.1128/JCM.00498-10
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Genetics And Genomics