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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF
Cellular Microbiology, Volume 13, No. 2, Year 2011
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Description
Plasmodium falciparum malaria is a major cause of morbidity and mortality in African children, and factors that determine the development of uncomplicated (UM) versus cerebral malaria (CM) are not fully understood. We studied the ex vivo responsiveness of microvascular endothelial cells to pro-inflammatory stimulation and compared the findings between CM and UM patients. In patients with fatal disease we compared the properties of vascular endothelial cells cultured from brain tissue to those cultured from subcutaneous tissue, and found them to be very similar. We then isolated, purified and cultured primary endothelial cells from aspirated subcutaneous tissue of patients with CM (ECCM) or UM (ECUM) and confirmed the identity of the cells before analysis. Upon TNF stimulation in vitro, ECCM displayed a significantly higher capacity to upregulate ICAM-1, VCAM-1 and CD61 and to produce IL-6 and MCP-1 but not RANTES compared with ECUM. The shedding of endothelial microparticles, a recently described parameter of severity in CM, and the cellular level of activated caspase-3 were both significantly greater in ECCM than in ECUM. These data suggest that inter-individual differences in the endothelial inflammatory response to TNF may be an additional factor influencing the clinical course of malaria. © 2010 Blackwell Publishing Ltd.
Authors & Co-Authors
Wassmer, Samuel Crocodile
Malawi, Zomba
University of Malawi
Moxon, Christopher Alan
Malawi, Zomba
University of Malawi
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Taylor, Terrie Ellen
United States, East Lansing
Msu College of Osteopathic Medicine
Malawi, Blantyre
Blantyre Malaria Project
Grau, Georges Emile Raymond
Australia, Sydney
The University of Sydney School of Medicine
Molyneux, Malcolm Edward
Malawi, Zomba
University of Malawi
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Craig, Alister Gordon
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Statistics
Citations: 68
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1111/j.1462-5822.2010.01528.x
ISSN:
14625814
e-ISSN:
14625822
Research Areas
Infectious Diseases
Maternal And Child Health