Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Shifts in microbial community functions and nitrifying communities as a result of combined application of copper and mefenoxam
FEMS Microbiology Letters, Volume 260, No. 1, Year 2006
Notification
URL copied to clipboard!
Description
In this microcosm study, we focused on the effect of a combined application of copper and mefenoxam on the functional diversity of soil microbial communities. Treatments with combined and separate applications of copper and mefenoxam were sampled at 24 and 60 days and control soil was sampled at 0, 24 and 60 days. Structural and metabolic profiling of microorganisms were performed by arbitrarily primed (AP) and RNA arbitrarily primed-PCR (RAP-PCR). Cluster analysis resulted in separate grouping of AP and RAP-PCR profiles, with differences between control and treatments being more pronounced with respect to RAP-PCR profiles. amoA, a functional molecular marker for β-subgroup ammonia-oxidizing bacteria, could only be detected at day 60 in treatments of mefenoxam, and mefenoxam+copper, with higher gene copies in the latter. There was also an increase in potential nitrification activity on application of mefenoxam and mefenoxam+copper. Comparison of amoA diversity was performed by denaturing gradient gel electrophoresis followed by construction of a clone library of amoA fragments amplified from the mefenoxam+copper-treated sample. Analysis of clones was performed by restriction digestion and subsequent sequencing. Patterns 1 and 5 were seen in 93% of the clones and clustered together with amoA sequences of Nitrosospira, indicating that Nitrosospira-like organisms are the major nitrifiers under mefenoxam treatments. © 2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved.
Authors & Co-Authors
Demanou, Joseph
Cameroon, Yaounde
Université de Yaoundé I
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Sharma, S.
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Weber, Anja
Germany, Berlin
Technische Universität Berlin
Wilke, Berndt Michael
Germany, Berlin
Technische Universität Berlin
Njiné, Thomas
Cameroon, Yaounde
Université de Yaoundé I
Monkiédjé, Adolphe
Cameroon, Yaounde
Université de Yaoundé I
Munch, Jean Charles
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Schloter, Michael
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Statistics
Citations: 27
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1111/j.1574-6968.2006.00299.x
ISSN:
03781097
e-ISSN:
15746968
Research Areas
Genetics And Genomics
Noncommunicable Diseases