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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Love-wave bacteria-based sensor for the detection of heavy metal toxicity in liquid medium
Biosensors and Bioelectronics, Volume 26, No. 4, Year 2010
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Description
The present work deals with the development of a Love-wave bacteria-based sensor platform for the detection of heavy metals in liquid medium. The acoustic delay-line is inserted in an oscillation loop in order to record the resonance frequency in real-time. A Polydimethylsiloxane (PDMS) chip with a liquid chamber is maintained by pressure above the acoustic wave propagation path.Bacteria (Escherichia coli) were fixed as bioreceptors onto the sensitive surface of the sensor coated with a polyelectrolyte (PE) multilayer using a simple and efficient layer-by-layer (LbL) electrostatic self-assembly procedure. Poly(allylamine hydrochloride) (PAH cation) and poly(styrene sulfonate) (PSS anion) were alternatively deposited so that the strong attraction between oppositely charged polyelectrolytes resulted in the formation of a (PAH-PSS)n-PAH molecular multilayer. The real-time characterization of PE multilayer and bacteria deposition is based on the measurement of the resonance frequency perturbation due to mass loading during material deposition. Real-time response to various concentrations of cadmium (Cd2+) and mercury (Hg2+) has been investigated. A detection limit as low as 10-12mol/l has been achieved, above which the frequency increases gradually up to 10-3mol/l, after a delay of 60s subsequent to their introduction onto bacterial cell-based biosensors. Beyond a 10-3mol/l a steep drop in frequency was observed. This response has been attributed to changes in viscoelastic properties, related to modifications in bacteria metabolism. © 2010 Elsevier B.V.
Authors & Co-Authors
Gammoudi, Ibtissèm
France, Bordeaux
Université de Bordeaux
Tunisia, Monastir
Faculté de Médecine de Monastir
Tarbague, Hakim
France, Bordeaux
Université de Bordeaux
Othmane, Ali
Tunisia, Monastir
Faculté de Médecine de Monastir
Moynet, Daniel
France, Bordeaux
Université de Bordeaux
Rebiére, Dominique
France, Bordeaux
Université de Bordeaux
Kalfat, R.
Tunisia, Sidi Thabet
Institut National de Recherche et D'analyse Physico-chimique
Dejous, Corinne
France, Bordeaux
Université de Bordeaux
Statistics
Citations: 54
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1016/j.bios.2010.07.118
ISSN:
09565663