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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Immunogenicity and immune modulatory effects of in silico predicted L. donovani candidate peptide vaccines
Human Vaccines and Immunotherapeutics, Volume 8, No. 12, Year 2012
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Description
Visceral leishmaniasis (VL) is a serious parasitic disease for which control measures are limited and drug resistance is increasing. First and second generation vaccine candidates have not been successful. The goal of the present study was to select possibly immunogenic L. donovani donovani GP63 peptides using immunoinformatics tools and to test their immunogenicity in vitro. The amino acid sequence of L. donovani donovani GP63 [GenBank accession: ACT31401] was screened using the EpiMatrix algorithm for putative T cell epitopes that would bind to the most common HLA class II alleles (DRB1&z.ast;1101 and DRB1&z.ast;0804) among at-risk populations. Four T cell epitopes were selected from nine potential candidates. Stimulation of whole blood from healthy volunteers using the peptides separately produced mean IFN-γ and IL-4 levels that were not significantly different from negative controls, while the pooled peptides produced a moderate IFN-γ increase in some volunteers. However, mean IL-10 levels were significantly reduced for all individuals compared with controls. The immunogenicity of these epitopes may be harnessed most effectively in a vaccine delivered in combination with immune-modulating adjuvants. © 2012 Landes Bioscience.
Authors & Co-Authors
Elfaki, Mona Eltahir
Sudan, Khartoum
Institute of Endemic Diseases Sudan
Khalil, Eltahir Awad Gasim
Sudan, Khartoum
Institute of Endemic Diseases Sudan
De Groot, Anne S.
United States, Providence
University of Rhode Island Feinstein Providence Campus
United States, Providence
Epivax Incorporated
Musa, Ahmed Mudawi
Sudan, Khartoum
Institute of Endemic Diseases Sudan
Gutierrez, Andres
United States, Providence
University of Rhode Island Feinstein Providence Campus
Younis, Brima Musa
Sudan, Khartoum
Institute of Endemic Diseases Sudan
Salih, Kawthar A.M.
Sudan, Khartoum
Elneil College of Medicine
el-Hassan, Ahmed Mohamed A.
Sudan, Khartoum
Institute of Endemic Diseases Sudan
Statistics
Citations: 38
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.4161/hv.21881
ISSN:
21645515
e-ISSN:
2164554X