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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
CD4
+
T cells spontaneously producing human immunodeficiency virus type I in breast milk from women with or without antiretroviral drugs
Retrovirology, Volume 8, Article 34, Year 2011
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Description
Background: Transmission of human immunodeficiency virus type 1 (HIV-1) through breast-feeding may involve both cell-free and cell-associated virus. This latter viral reservoir remains, however, to be fully explored. CD4+ T cell-associated virus production in breast milk was therefore investigated.Methods: The ex vivo spontaneous production of HIV-1 antigen and HIV-1 RNA by CD4+ T cells was measured in paired blood and breast milk samples from 15 HIV-1 infected women treated or not with antiretroviral drugs. Spontaneous antigen secreting cells (HIV-1-AgSCs) from breast milk and blood were enumerated by an ELISpot assay, and cell-associated HIV-1 RNA was quantified by real-time PCR in supernatants of CD4+ T cells cultured for 18 hours without addition of polyclonal activators.Results: Among the CD4+ T cells present in breast milk, memory cells expressing high levels of cell-surface activation markers were predominant. Spontaneous HIV-1-AgSCs were detected and enumerated in the breast milk of all 15 women, with a median number of 13.0 and 9.5 HIV-1- AgSCs/106 CD4+ T cells in aviremic (n = 7) and viremic (n = 8) women, respectively. Cell- associated HIV-1 RNA was detected in cell-free supernatants from 4/7 aviremic and 5/8 viremic individuals at median levels of 190 and 245 copies/ml, respectively.Conclusions: Activated CD4+ T cells producing HIV-1 are detected in the breast milk of untreated individuals as well as those receiving highly active antiretroviral therapy. This finding strongly suggests that HIV-1 replication occurs in latently infected CD4+ T cells that, upon spontaneous activation, revert to productively infected cells. These cells might be responsible for a residual breast milk transmission despite maternal highly active antiretroviral therapy. © 2011 Valea et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Valéa, Diane C.
Burkina Faso, Ouagadougou
Centre Muraz
Burkina Faso
Institut de Recherche en Sciences de la Santé/dro
Tuaillon, Édouard
France, Montpellier
Université de Montpellier
France, Montpellier
Institut Pour la Recherche en Biothérapie
France, Montpellier
Hopital Lapeyronie
Al Tabaa, Yassine
France, Montpellier
Université de Montpellier
France, Montpellier
Institut Pour la Recherche en Biothérapie
Rouet, François
Burkina Faso, Ouagadougou
Centre Muraz
Rubbo, Pierre Alain
France, Montpellier
Université de Montpellier
France, Montpellier
Institut Pour la Recherche en Biothérapie
Méda, Nicolas Yelbomkan
Burkina Faso
Institut de Recherche en Sciences de la Santé/dro
Foulongne, Vincent
France, Montpellier
Université de Montpellier
France, Montpellier
Hopital Lapeyronie
Bolloré, Karine
France, Montpellier
Université de Montpellier
France, Montpellier
Institut Pour la Recherche en Biothérapie
Nagot, Nicolas
France, Montpellier
Université de Montpellier
van de Perre, Philippe
France, Montpellier
Université de Montpellier
France, Montpellier
Hopital Lapeyronie
Vendrell, Jean Pierre
France, Montpellier
Université de Montpellier
France, Montpellier
Institut Pour la Recherche en Biothérapie
France, Montpellier
Hopital Lapeyronie
Statistics
Citations: 56
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1186/1742-4690-8-34
Research Areas
Infectious Diseases
Maternal And Child Health
Participants Gender
Female