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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
In vitro cellular and humoral responses to Schistosoma mansoni vaccine candidate antigens
Acta Tropica, Volume 88, No. 2, Year 2003
Notification
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Description
Few studies comparing schistosomiasis vaccine candidate antigens between laboratories have been carried out and published. Generally, only the investigators who discovered the molecules have evaluated them in either experimental animal models or in human correlate studies. In an attempt to identify responses against specific antigens and investigate their association with resistance versus susceptibility to re-infection, we studied the serological reactions and the cytokine responses stimulated by a panel of 10 candidate vaccine molecules in 225 long-term residents of an area endemic for Schistosoma mansoni in Egypt. The panel consisted of four recombinant antigens (Sm62-Irv5, Sm37-G3PDH, Sm28-GST and Sm14-FABP), one full-length native protein (Sm97-paramyosin), two synthetic peptides (MAP3 and MAP4) and three unpublished antigens (PR52-filamin, PL45-phosphoglycerate kinase, PN18-cyclophilin). Two different study designs, one based on retrospective and the other on prospective parasitological data were applied in the evaluation of the immune responses. Using historical data collected over the previous 5 years, correlations between frequency of re-infection and antigen-specific immune responses were investigated. In the prospective arm of the study, the subjects were followed over time after treatment with praziquantel with periodic immunological tests and stool examinations. Thus, highly specific humoral and cellular immune reactions in response to the 10 antigens described above could be correlated, both prospectively and retrospectively, with detailed epidemiological data covering a 66-month period. The immune response profiles produced were unique to each antigen but no clear "winner" or "winners" were identified. However, markers for both resistance and susceptibility to re-infection were identified for each molecule indicating which types of responses to aim for in vaccination and which ones to avoid. The insights gained from this approach should be useful for antigen selection and ultimately for vaccine formulation prior to Phase I/II trials in humans. © 2003 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Al-Sherbiny, Maged Mustafa
Egypt, Giza
Faculty of Science
Osman, Ahmed M.
Egypt, Giza
Faculty of Science
Barakat, Rashida M.R.
Egypt, Alexandria
High Institute of Public Health
El-Morshedy, Hala Nasser
Egypt, Alexandria
High Institute of Public Health
Bergquist, Robert N.
Switzerland, Geneva
Organisation Mondiale de la Santé
Olds, Richard
United States, Milwaukee
Medical College of Wisconsin
Statistics
Citations: 95
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/S0001-706X(03)00195-5
ISSN:
0001706X
Research Areas
Infectious Diseases
Study Design
Cohort Study
Study Locations
Egypt