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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress
Journal of Plant Physiology, Volume 165, No. 5, Year 2008
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Description
Salt stress is one of the major abiotic stresses in agriculture worldwide, especially in the Mediterranean area. We report here a proteomic approach to investigate the salt stress-responsive proteins in grapevine (Vitis vinifera). Two-dimensional electrophoresis (2-DE) was used to analyze the proteome of the salt-tolerant Tunisian grapevine cultivar Razegui, subjected to a supply of 100 mm NaCl over 15 d. Analysis of 2-DE gels derived from stressed plants revealed more than 800 reproducibly detected protein spots, with 48 proteins displaying a differential expression pattern, including 32 up-regulated, 9 down-regulated and 7 new protein spots induced after salt treatment. The presence of stress-responsive proteins in the different plant organs suggests that salt spreads systemically. Edman degradation analysis and database searching aided us in identifying a major protein GP. Database analysis revealed that this peptide has a 98% sequence similarity with a pathogenesis-related (PR) protein 10 (V. vinifera). A full-length cDNA encoding the GP protein was isolated from grapevine salt-stressed leaves and sequenced. The predicted protein contained 158 amino acids and showed 98% identity with PR10 protein of of V. vinifera (accession no. Cac16165). © 2007 Elsevier GmbH. All rights reserved.
Authors & Co-Authors
Jellouli, Neila
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Ben Jouira, Hatem
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Skouri, Houda
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Ghorbel, Abdelwahed
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Gourgouri, Ali
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Mliki, A.
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Statistics
Citations: 84
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1016/j.jplph.2007.02.009
ISSN:
01761617
Research Areas
Genetics And Genomics