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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Countrywide spread of community- and hospital-acquired extended-spectrum β-lactamase (CTX-M-15)-producing Enterobacteriaceae in Lebanon
Journal of Clinical Microbiology, Volume 43, No. 7, Year 2005
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Description
A prospective study was carried out to assess the extent of carriage of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae at both hospital and community levels in Lebanon. A total of 1,442 fecal samples were collected from hospital-based patients and 58 from health care workers of six Lebanese tertiary care general hospitals located in different areas of Lebanon between January and March 2003. A total of 382 fecal samples were also collected from healthy subjects between April and June 2003. The samples analysis led to the identification of 118 strains as ESBL producers based on the synergistic effects between clavulanate and selected β-lactams (ceftazidime and cefotaxime). These strains were isolated from 72 subjects: 61 patients, 2 health care workers, and 9 healthy subjects. One representative strain per subject was selected, and a total of 72 nonduplicate ESBL producers, including a high majority of Escherichia coli (n = 56), Klebsiella pneumoniae (n = 9), Enterobacter cloacae (n = 6), and Citrobacter freundii (n = 1), were characterized. The molecular analysis revealed that the majority of the strains (83%) express CTX-M-15 ESBL (pI 8.6). SHV-5a ESBL (pI 8.2) was produced by 18% of the strains. DNA macrorestriction analysis of ESBL-producing E. coli presented 38 different genotypes, revealing the absence of clonal link among these strains. In addition to the fact that the present study highlights the emergence and the countrywide dissemination of CTX-M-15-producing E. coli in Lebanon, it represents the first report of an SHV-5a-producing C. freundii. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Ayoub Moubareck, Carole
France, Paris
Faculté Des Sciences Pharmaceutiques et Biologiques
Daoud, Ziad D.
Lebanon, Al Koura
University of Balamand
Irani-Hakimé, Noha A.
Lebanon, Al Koura
University of Balamand
Hamzé, Monzer M.
Lebanon, Beirut
Université Libanaise
Lebanon, Tripol
Nini Hospital
Mangeney, Nicole
France, Paris
Faculté Des Sciences Pharmaceutiques et Biologiques
Matta, Hiam
Lebanon, Beirut
Arz Hospital
Mokhbat, Jacques Emile
Lebanon, Beirut
Lebanese American University Medical Center-rizk Hospital
Rohban, Raymond
Lebanon, Beirut
Saint Joseph Hospital, Beirut
Karam Sarkis, Dolla
Lebanon, Beirut
Hôtel-dieu de France Hospital
Doucet-Populaire, Florence
France, Paris
Faculté Des Sciences Pharmaceutiques et Biologiques
France, Versailles
Chv Centre Hospitalier de Versailles
Statistics
Citations: 157
Authors: 10
Affiliations: 9
Identifiers
Doi:
10.1128/JCM.43.7.3309-3313.2005
ISSN:
00951137
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Cohort Study