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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Trypanosoma (Nannomonas) congolense: Molecular characterization of a new genotype from Tsavo, Kenya
Parasitology, Volume 106, No. 2, Year 1993
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Description
Trypanosoma (Nannomonas) congolense comprises morphologically identical but genetically heterogeneous parasites infective to livestock and other mammalian hosts; three different genotypes of this parasite have been described previously. Restriction enzyme fragment length polymorphisms (RFLPs) in both kinetoplast DNA minicircle and nuclear DNA sequences, and randomly amplified polymorphic deoxyribonucleic acid (RAPD) patterns have been used here to demonstrate the existence of another type of T. (N.) congolense that is genotypically distinct from those that have so far been characterized at the molecular level. A highly repetitive, tandemly arranged DNA sequence and oligonucleotide primers, for use in polymerase chain reaction (PCR) amplification are described, which can be used for specific identification of the trypanosome and its distinction from others within the Nannomonas subgenus. © 1993, Cambridge University Press. All rights reserved.
Authors & Co-Authors
Majiwa, Phelix O.A.
Kenya, Nairobi
International Centre of Insect Physiology and Ecology Nairobi
Maina, M.
Kenya, Nairobi
International Centre of Insect Physiology and Ecology Nairobi
Waitumbi, John Njenga
Kenya, Nairobi
International Centre of Insect Physiology and Ecology Nairobi
Mihok, Steve
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Zweygarth, Erich P.
Kenya
Kenya Trypanosomiasis Research Institute Ketri
Statistics
Citations: 108
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1017/S0031182000074941
e-ISSN:
14698161
Research Areas
Cancer
Genetics And Genomics
Study Locations
Kenya