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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Characterization of ten date palm (Phoenix dactylifera L.) cultivars from Saudi Arabia using AFLP and ISSR markers
Comptes Rendus - Biologies, Volume 337, No. 1, Year 2014
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Description
Date palm is the most economically important plant in the Middle East due to its nutritionally valuable fruit. The development of accurate DNA fingerprints to characterize cultivars and the detection of genetic diversity are of great value for breeding programs. The present study explores the usefulness of ISSR and AFLP molecular markers to detect relationships among 10 date palm (Phoenix dactylifera L.) cultivars from Saudi Arabia. Thirteen ISSR primers and six AFLP primer combinations were examined. The level of polymorphism among cultivars for ISSRs ranged from 20% to 100% with an average of 85%. Polymorphism levels for AFLPs ranged from 63% to 84% with an average of 76%. The total number of cultivar-specific markers was 241, 208 of which were generated from AFLP analysis. AJWA cultivar had the highest number of cultivar-specific ISSR markers, whereas DEK, PER, SUK-Q, SHA and MOS-H cultivars had the lowest. RAB and SHA cultivars had the most and least AFLP cultivar-specific markers, respectively. The highest pairwise similarity indices for ISSRs, AFLPs and combined markers were 84% between DEK (female) and PER (female), 81% between SUK-Q (male) and RAB (male), and 80% between SUK-Q (male) and RAB (male), respectively. The lowest similarity indices were 65% between TAB (female) and SUK-Q (male), 67% between SUK-A (female) and SUK-Q (male), and 67% between SUK-A (female) and SUK-Q (male). Cultivars of the same sex had higher pairwise similarities than those between cultivars of different sex. The Neighbor-Joining (NJ) tree generated from the ISSR dataset was not well resolved and bootstrap support for resolved nodes in the tree was low. AFLP and combined data generated completely resolved trees with high levels of bootstrap support. In conclusion, AFLP and ISSR approaches enabled discrimination among 10 date palm cultivars of from Saudi Arabia, which will provide valuable information for future improvement of this important crop. © 2013 Académie des sciences.
Authors & Co-Authors
Sabir, Jamal S.M.
Saudi Arabia, Jeddah
King Abdulaziz University
Abo-Aba, S. E.M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
National Research Centre
Bafeel, Sameera Omar
Saudi Arabia, Jeddah
King Abdulaziz University
Zari, Talal A.
Saudi Arabia, Jeddah
King Abdulaziz University
Edris, S. A.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Cairo
College of Agriculture
Shokry, Ahmed M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
Agricultural Genetic Engineering Research Institute Agerl
Atef, Ahmed
Saudi Arabia, Jeddah
King Abdulaziz University
Gadalla, Nour O.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
National Research Centre
Ramadan, Ahmed M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
Agricultural Genetic Engineering Research Institute Agerl
Al-Kordy, Magdy A.A.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
National Research Centre
El-Domyati, Fotouh M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Cairo
College of Agriculture
Jansen, Robert K.
Saudi Arabia, Jeddah
King Abdulaziz University
United States, Austin
The University of Texas at Austin
Bahieldin, Ahmed M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Cairo
College of Agriculture
Statistics
Citations: 28
Authors: 13
Affiliations: 5
Identifiers
Doi:
10.1016/j.crvi.2013.11.003
ISSN:
16310691
e-ISSN:
17683238
Research Areas
Genetics And Genomics
Participants Gender
Male
Female