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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Downregulation of microRNA-24 and -181 parallels the upregulation of IFN-γ secreted by activated human CD4 lymphocytes
Human Immunology, Volume 75, No. 7, Year 2014
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Description
IFN-γ is a cytokine with important roles in the innate and adaptive immune responses. This cytokine is secreted by activated T cells, NK cells and macrophages. Studies on the regulation of human IFN-γ expression had been previously focused on the promoter region. Consequently, the role of microRNAs (miRs) in this regulation has not been investigated yet. As miR-24 and miR-181 were found to have potential target sites in IFN-γ mRNA 3'UTR, we assessed their impact on IFN-γ expression by co-stimulating PB CD4+ T cells with anti-CD3, anti-CD28, IL-12, and IL-18. This co-stimulation cocktail induced an abundant secretion of IFN-γ together with a down-regulation of miR-24, and miR-181. Existence of a link between these two phenomena was further substantiated by transfection and transduction assays that showed that these two miRs negatively regulate IFN-γ expression by directly binding to their target sites in the mRNA. Thus, identifying target sites for miR-24 and miR-181 in IFN-γ-3'UTR points to a novel regulatory mechanism of this crucial gene. © 2014 American Society for Histocompatibility and Immunogenetics.
Authors & Co-Authors
Fayyad-kazan, Hussein
Belgium, Brussels
Institut Jules Bordet
Hamade, Eva
Lebanon, Beirut
Université Libanaise
Rouas, Rédouane
Belgium, Brussels
Institut Jules Bordet
Najar, Mehdi
Belgium, Brussels
Hôpital Erasme
Fayyad-Kazan, Mohammad
Belgium, Brussels
Université Libre de Bruxelles
El-Zein, Nabil
Lebanon, Beirut
Université Libanaise
ElDirani, Rim
Lebanon, Beirut
Université Libanaise
Hussein, Nader
Lebanon, Beirut
Université Libanaise
Fakhry, Maya
Lebanon, Beirut
Université Libanaise
Al-Akoum, Carine
Lebanon, Beirut
Université Libanaise
Burny, Arséne
Belgium, Brussels
Institut Jules Bordet
Martiat, Philippe
Belgium, Brussels
Institut Jules Bordet
Badran, Bassam M.
Lebanon, Beirut
Université Libanaise
Statistics
Citations: 13
Authors: 13
Affiliations: 4
Identifiers
Doi:
10.1016/j.humimm.2014.01.007
ISSN:
01988859
e-ISSN:
18791166
Research Areas
Genetics And Genomics