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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemistry
Cytotoxicity mechanisms of nitrogen-doped graphene obtained by electrochemical exfoliation of graphite rods, on human endothelial and colon cancer cells
Carbon, Volume 158, Year 2020
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Description
Three nitrogen-doped graphene samples were synthesized by electrochemical exfoliation of graphite rods via pulses of current. Depending on the synthesis conditions, the samples had different content of nitrogen (0.79, 2.56 and 2.33 wt%) and were denoted as NGr-1, NGr-2 and NGr-3, respectively. All samples consist in a mixture of graphene oxide, few- and multi-layer graphene. Several biological effects (cytotoxicity, oxidative stress induction, apoptosis, autophagy and DNA lesions) induced by the N-doped graphenes on human endothelial and colon cancer cells were investigated. The in vitro effects of graphenes were different and depended on the type of cell and the amount of nitrogen in the structure. On human endothelial cells, the graphenes showed antioxidant effects and were less toxic than on colon cancer cells, especially the one with low content of nitrogen (NGr-1). In contrast, on colon cancer cells the graphenes induced cell death by various mechanisms: free radicals generation, apoptosis, autophagy and DNA damage. The most severe DNA lesions and ultrastructural changes were produced by graphene with high content of nitrogen (NGr-2 and NGr-3). Our research demonstrates the cytotoxic effect of graphene with high content of nitrogen on colon cancer cells and antioxidant and protective properties on human endothelial cells. © 2019 Elsevier Ltd
Authors & Co-Authors
Olteanu, Diana
Romania, Cluj Napoca
Universitatea de Medicina si Farmacie Iuliu Hatieganu Din Cluj-napoca
Coroş, Maria
Romania, Cluj Napoca
National Institute for Research and Development of Isotopic and Molecular Technologies
Suciu, Maria
Romania, Cluj Napoca
National Institute for Research and Development of Isotopic and Molecular Technologies
Romania, Cluj Napoca
Universitatea Babeș-bolyai
Cenariu, Mihai C.
Romania, Cluj Napoca
Universitatea de Stiinte Agricole si Medicina Veterinara Din Cluj-napoca
Stefan-van Staden, Raluca Ioana
Romania, Bucharest
National Institute of Research for Electrochemistry and Condensed Matter
Romania, Bucharest
University Politehnica of Bucharest
Pruneanu, Stela Maria
Romania, Cluj Napoca
National Institute for Research and Development of Isotopic and Molecular Technologies
Statistics
Citations: 20
Authors: 6
Affiliations: 6
Identifiers
Doi:
10.1016/j.carbon.2019.12.011
ISSN:
00086223
Research Areas
Cancer
Environmental
Genetics And Genomics