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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Identification of the major functional proteins of prokaryotic lipid droplets
Journal of Lipid Research, Volume 53, No. 3, Year 2012
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Description
Storage of cellular triacylglycerols (TAGs) in lipid droplets (LDs) has been linked to the progression of many metabolic diseases in humans, and to the development of biofuels from plants and microorganisms. However, the biogenesis and dynamics of LDs are poorly understood. Compared with other organisms, bacteria seem to be a better model system for studying LD biology, because they are relatively simple and are highly efficient in converting biomass to TAG. We obtained highly purified LDs from Rhodococcus sp. RHA1, a bacterium that can produce TAG from many carbon sources, and then comprehensively characterized the LD proteome. Of the 228 LD-associated proteins identified, two major proteins, ro02104 and PspA, constituted about 15% of the total LD protein. The structure predicted for ro02104 resembles that of apolipoproteins, the structural proteins of plasma lipoproteins in mammals. Deletion of ro02104 resulted in the formation of supersized LDs, indicating that ro02104 plays a critical role in cellular LD dynamics. The putative α helix of the ro02104 LD-targeting domain (amino acids 83-146) is also similar to that of apolipoproteins. We report the identification of 228 proteins in the proteome of prokaryotic LDs, identify a putative structural protein of this organelle, and suggest that apolipoproteins may have an evolutionarily conserved role in the storage and trafficking of neutral lipids. Copyright © 2012 by the American Society for Biochemistry and Molecular Biology, Inc.
Authors & Co-Authors
Ding, Yunfeng
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
China, Beijing
University of Chinese Academy of Sciences
Yang, Li
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
China, Beijing
University of Chinese Academy of Sciences
Zhang, Shuyan
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
Wang, Yang
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
China, Beijing
University of Chinese Academy of Sciences
Du, Yalan
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
China, Hengyang
University of South China
Pu, Jing
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
China, Beijing
University of Chinese Academy of Sciences
Peng, Gong
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
China, Beijing
University of Chinese Academy of Sciences
Chen, Yong
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
Zhang, Huina
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
Yu, Jinhai
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
China, Beijing
University of Chinese Academy of Sciences
Hang, Haiying
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
Wu, Peng
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
Yang, Fuquan
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
Yang, Hongyuan
Australia, Sydney
Unsw Sydney
Steinbüchel, Alexander
Germany, Munster
University of Münster
Saudi Arabia, Jeddah
King Abdulaziz University
Liu, Pingsheng
China, Beijing
Institute of Biophysics Chinese Academy of Sciences
Statistics
Citations: 102
Authors: 16
Affiliations: 6
Identifiers
Doi:
10.1194/jlr.M021899
ISSN:
00222275
e-ISSN:
15397262
Research Areas
Environmental
Noncommunicable Diseases