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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Serial analysis of gene expression in the southern cattle tick following acaricide treatment of larvae from organophosphate resistant and susceptible strains
Insect Molecular Biology, Volume 16, No. 1, Year 2007
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Description
Organophosphate resistant and susceptible tick larvae from laboratory strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus were exposed to low doses of the organophosphate (OP) acaricide, coumaphos. Serial analysis of gene expression (SAGE) was used to analyse differential gene expression in response to OP treatment and to compare the responses of OP-treated and -untreated resistant and susceptible tick larvae. An R. microplus Gene Index was used as an EST database to identify genes which corresponded to SAGE tags whose abundance changed in response to acaricide exposure. Relative quantitative RT-PCR was used to confirm the differential expression results from the SAGE experiments. Of particular interest is a SAGE tag which corresponds to a cytochrome P450-like EST in the Gene Index which was more abundant in untreated OP resistant larvae compared to untreated OP susceptible larvae. This SAGE tag was also more abundant in OP resistant larvae treated with OP compared to OP susceptible larvae treated with OP. © 2007 The Authors Journal compilation © 2007 The Royal Entomological Society.
Authors & Co-Authors
Guerrero, Felix David
United States, Washington, D.c.
Usda Agricultural Research Service
United States, Washington, D.c.
United States Department of Agriculture
Bendele, Kylie G.
United States, Washington, D.c.
Usda Agricultural Research Service
Li, Andrew Yongsheng
United States, Washington, D.c.
Usda Agricultural Research Service
Miller, Robert John
United States, Washington, D.c.
Usda Agricultural Research Service
Pleasance, Erin D.
Canada, Vancouver
Canada‘s Michael Smith Genome Sciences Centre
Varhol, Richard J.
Canada, Vancouver
Canada‘s Michael Smith Genome Sciences Centre
Nene, Vishvanath M.
United States, Rockville
J. Craig Venter Institute
Statistics
Citations: 24
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1111/j.1365-2583.2007.00699.x
ISSN:
13652583
Research Areas
Genetics And Genomics
Study Approach
Quantitative