Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Environmental surveillance of wild poliovirus circulation in Egypt - Balancing between detection sensitivity and workload
Journal of Virological Methods, Volume 126, No. 1-2, Year 2005
Notification
URL copied to clipboard!
Description
Examination of sewage specimens for poliovirus (environmental surveillance) was adopted as a supplementary tool in the surveillance of poliomyelitis in Egypt. Sewage samples were concentrated about 50-fold using a simple two-phase separation technique, and inoculated in cell cultures in two collaborating laboratories in parallel. All but 9 of the 293 (97%) samples collected from January 2001 to December 2002 contained poliovirus and/or other enteroviruses, with polioviruses being detected in 84% of the samples. The proportion of specimens containing type 1 wild poliovirus (PV1W, the North-East African (NEAF) genotype) was less in 2002 (16%) than in 2001 (57%), and further decreased in 2003. While the overall sensitivity to detect PV1W was similar in the two collaborating laboratories, the specimens scored positive were not identical. Parallel cultures inoculated with aliquots of a given specimen very frequently resulted in isolation of different viruses. Moreover, partial sequence analysis occasionally revealed representatives of different genetic lineages of PV1W in a given specimen. These results emphasize the need to use intensive laboratory analysis to optimise sample sensitivity in environmental poliovirus surveillance, and the difficulties in reproducing the isolation results by simple re-inoculation of samples containing a mixture of different viruses. © 2005 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Hovi, Tapani
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Blomqvist, Soile
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Nasr, Eman
Egypt, Cairo
Egyptian Organization for Biological and Vaccine Production
Burns, Cara Carthel
United States, Atlanta
Centers for Disease Control and Prevention
Sarjakoski, Tarja
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Ahmed, Nahed
Egypt, Cairo
Egyptian Organization for Biological and Vaccine Production
Savolainen, Carita
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Roivainen, Merja M.
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Stenvik, Mirja
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Laine, Pia
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Barakat, Ibrahim
Egypt, Cairo
Egyptian Ministry of Health and Population
Wahdan, Mohammed Helmy
Switzerland, Geneva
Organisation Mondiale de la Santé
Kamel, Faten A.
Switzerland, Geneva
Organisation Mondiale de la Santé
Asghar, Humayun
Switzerland, Geneva
Organisation Mondiale de la Santé
Pallansch, Mark A.
United States, Atlanta
Centers for Disease Control and Prevention
Kew, Olen M.
United States, Atlanta
Centers for Disease Control and Prevention
Gary, Howard E.
United States, Atlanta
Centers for Disease Control and Prevention
DeGourville, Esther M.
Switzerland, Geneva
Organisation Mondiale de la Santé
El Bassioni, Laila
Egypt, Cairo
Egyptian Organization for Biological and Vaccine Production
Statistics
Citations: 49
Authors: 19
Affiliations: 5
Identifiers
Doi:
10.1016/j.jviromet.2005.02.002
ISSN:
01660934
Research Areas
Genetics And Genomics
Study Locations
Egypt