Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Cross-clade neutralizing activity of human anti-V3 monoclonal antibodies derived from the cells of individuals infected with non-B clades of human immunodeficiency virus type 1
Journal of Virology, Volume 80, No. 14, Year 2006
Notification
URL copied to clipboard!
Description
The majority of global human immunodeficiency virus infections are caused by viruses characterized by a GPGQ motif at the tip of the V3 loop. Characterization of anti-V3 monoclonal antibodies (MAbs) that neutralize isolates with the GPGQ V3 motif is an important step in designing vaccines that will induce such Abs. Consequently, seven human anti-V3 MAbs derived from the cells of individuals infected with non-B-subtype viruses (anti-V3 non-B MAbs) were generated from the cells of individuals from Africa infected with circulating recombinant forms CRF02_AG, CRF09_cpx, and CRF13_cpx, each of which contains a subtype A env gene. Sequence analysis of plasma viruses revealed a GPGQ motif at the apex of the V3 loop from six of the seven subjects and a GPGR motif from one subject. The MAbs were selected with fusion proteins (FP) containing V392UG037.8 or V3JR-CSF from subtype A or B, respectively. In virus binding assays, five of the seven (71%) anti-V3 non-B MAbs bound to V3-FPs from both subtype A and subtype B, while only four of the nine (44%) anti-V3B MAbs recognized both V3-FPs. Using two neutralization assays, both the anti-V3non-B and the anti-V3B MAbs neutralized subtype B viruses with similar activities, while the anti-V3non-B MAbs exhibited a tendency toward both increased potency and breadth of neutralization against non-B viruses compared to anti-V3B MAbs. Statistical significance was not achieved, due in large measure to the sizes of the MAb panels, but the overall pattern of data strongly suggests that viruses with the GPGQ motif at the tip of the V3 loop induce anti-V3 Abs with broader cross-neutralizing activity than do viruses with the GPGR motif. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Górny, Miroslaw K.
United States, New York
Nyu Grossman School of Medicine
Williams, Constance A.U.
United States, New York
Nyu Grossman School of Medicine
Volsky, Barbara
United States, New York
Nyu Grossman School of Medicine
Revesz, Kathy
United States
Va Medical Center
Wang, Xiaohong
United States
Va Medical Center
Burda, Sherri T.
United States, New York
Nyu Grossman School of Medicine
Kimura, Tetsuya
United States, New York
Nyu Grossman School of Medicine
Konings, Frank A.J.
United States, New York
Nyu Grossman School of Medicine
Nádas, Arthur J.
United States, New York
Nyu Grossman School of Medicine
Anyangwe, Christopher A.
Cameroon
Alpha Royal Clinic
Nyambi, Phillipe N.
United States, New York
Nyu Grossman School of Medicine
United States
Va Medical Center
Krachmarov, Chavdar
United States, Newark
Public Health Research Institute
Pinter, Abraham
United States, Newark
Public Health Research Institute
Zolla Pazner, Susan B.
United States, New York
Nyu Grossman School of Medicine
United States
Va Medical Center
Statistics
Citations: 105
Authors: 14
Affiliations: 4
Identifiers
Doi:
10.1128/JVI.02202-05
ISSN:
0022538X
Research Areas
Genetics And Genomics