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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Role of the interleukin 10 family of cytokines in patients with immune reconstitution inflammatory syndrome associated with HIV infection and tuberculosis
Journal of Infectious Diseases, Volume 207, No. 7, Year 2013
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Description
Background. The interleukin 10 (IL-10) family comprises cytokines structurally related to IL-10 that share signaling receptors that have conserved signaling cascades. The immunopathogenesis of immune reconstitution inflammatory syndrome (IRIS) in patients with human immunodeficiency virus (HIV) infection and tuberculosis remains incompletely understood. We hypothesized that a deficiency of IL-10 and its homologs may contribute to the immunopathology of IRIS in these patients.Methods. We performed a case-control analysis involving patients with HIV infection and tuberculosis who had IRIS at clinical presentation (tuberculosis-IRIS) and similar patients with HIV infection and tuberculosis who did not develop tuberculosis-IRIS (non-IRIS). Peripheral blood mononuclear cells (PBMCs) were cultured in the presence or absence of heat-killed Mycobacterium tuberculosis for 6 and 24 hours. Messenger RNA was analyzed by quantitative reverse transcription polymerase chain reaction analysis. Cytokine concentrations in serum were also determined.Results. Cultures of PBMCs stimulated with M. tuberculosis for 24 hours yielded higher IL-10 and interleukin 22 (IL-22) transcript levels for tuberculosis-IRIS patients, compared with non-IRIS patients. Analysis of corresponding serum samples showed significantly higher concentrations of IL-10 and IL-22 in tuberculosis-IRIS patients, compared with non-IRIS patients.Conclusions. IL-10 and IL-22 were differentially induced in PBMCs from tuberculosis-IRIS patients after in vitro stimulation, and higher concentrations of their corresponding proteins were detected in serum (in vivo). The higher levels of IL-10 observed in this study may represent a compensatory antiinflammatory response during tuberculosis-IRIS. The elevated levels of IL-22 suggest an association between this cytokine and immunopathology during tuberculosis-IRIS. © 2013 The Author.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3583273/bin/supp_207_7_1148__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3583273/bin/supp_jit002_jit002supp.docx
Authors & Co-Authors
Tadokera, Rebecca
South Africa, Cape Town
University of Cape Town
Andrea Wilkinson, Katalin Andrea
South Africa, Cape Town
University of Cape Town
United Kingdom, London
Mrc National Institute for Medical Research
Meintjes, Graeme Ayton
South Africa, Cape Town
University of Cape Town
South Africa
G. F. Jooste Hospital
United Kingdom, London
Imperial College London
Skolimowska, Keira H.
South Africa, Cape Town
University of Cape Town
Matthews, Kerryn
South Africa, Cape Town
University of Cape Town
Seldon, Ronnett
South Africa, Cape Town
University of Cape Town
Rangaka, Molebogeng Xheeda
South Africa, Cape Town
University of Cape Town
Maartens, Gary Tuberculosis
South Africa, Cape Town
University of Cape Town
South Africa
G. F. Jooste Hospital
Wilkinson, Robert J.
South Africa, Cape Town
University of Cape Town
South Africa
G. F. Jooste Hospital
United Kingdom, London
Imperial College London
United Kingdom, London
Mrc National Institute for Medical Research
Statistics
Citations: 30
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1093/infdis/jit002
ISSN:
00221899
Research Areas
Infectious Diseases
Study Design
Case-Control Study
Study Approach
Quantitative