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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Identification of informative metabolic responses using a minibioreactor: A small step change in the glucose supply rate creates a large metabolic response in Saccharomyces cerevisiae
Yeast, Volume 29, No. 3-4, Year 2012
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Description
In this study, a previously developed mini-bioreactor, the Biocurve, was used to identify an informative stimulus-response experiment. The identified stimulus-response experiment was a modest 50% shift-up in glucose uptake rate (qGLC) that unexpectedly resulted in a disproportionate transient metabolic response. The 50% shift-up in qGLC in the Biocurve resulted in a near tripling of the online measured oxygen uptake (qO 2) and carbon dioxide production (qCO 2) rates, suggesting a considerable mobilization of glycogen and trehalose. The 50% shift-up in qGLC was subsequently studied in detail in a conventional bioreactor (4l working volume), which confirmed the results obtained with the Biocurve. Especially relevant is the observation that the 50% increase in glucose uptake rate led to a three-fold increase in glycolytic flux, due to mobilization of storage materials. This explains the unexpected ethanol and acetate secretion after the shift-up, in spite of the fact that after the shift-up the qGLC was far less than the critical value. Moreover, these results show that the correct in vivo fluxes in glucose pulse experiments cannot be obtained from the uptake and secretion rates only. Instead, the storage fluxes must also be accurately quantified. Finally, we speculate on the possible role that the transient increase in dissolved CO 2 immediately after the 50% shift-up in qGLC could have played a part in triggering glycogen and trehalose mobilization. © 2012 John Wiley & Sons, Ltd.
Authors & Co-Authors
Aboka, Fredrick O.
Netherlands, Leiden
Octoplus nv
van Winden, Wouter A.
Netherlands, Delft
Dsm Biotechnology Center
Reginald, Mashego M.
South Africa, Pretoria
The Council for Scientific and Industrial Research
van Gulik, Walter M.
Netherlands, Delft
Delft University of Technology
van de Berg, Marco
Netherlands, Delft
Dsm Biotechnology Center
Oudshoorn, Arthur
Netherlands, Delft
Applikon Biotechnology B.v.
Heijnen, Sef J.
Netherlands, Delft
Delft University of Technology
Statistics
Citations: 9
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1002/yea.2892
ISSN:
0749503X
e-ISSN:
10970061
Research Areas
Environmental
Noncommunicable Diseases
Study Approach
Quantitative