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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Quantitative determination of fumonisins bi and b
2
by high-performance liquid chromatography with fluorescence detection
Journal of Liquid Chromatography, Volume 13, No. 10, Year 1990
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Description
A high-performance liquid chromatographic method has been developed for the quantitative determination of the recently described mycotoxins, fumonisins B1 (FB1) and B2 (FB2) utilizing pre-column derivatization with o-phthaldialdehyde, isocratic elution, and fluorescence detection. Prior to analysis, sample extracts were purified on strong anion exchange cartridges. The method has been applied to the analysis of naturally contaminated corn and mixed horse feed samples as well as fungal culture material, for the presence of the mycotoxins. Detection limits are approximately 50 ng g-1 for FB1 and 100 ng g-1 for FB2. The method proved to be highly reproducible and recoveries of the toxins from the purification steps were found to be 99.5% and 85.9% for FB1 and FB2, respectively. © 1990, Taylor & Francis Group, LLC. All rights reserved.
Authors & Co-Authors
Shephard, Gordon Seymour
South Africa, Tygerberg
South African Medical Research Council
Sydenham, Erick W.
South Africa, Tygerberg
South African Medical Research Council
Thiel, Pieter G.
South Africa, Tygerberg
South African Medical Research Council
Gelderblom, Wentzel C.A.
South Africa, Tygerberg
South African Medical Research Council
Statistics
Citations: 453
Authors: 4
Affiliations: 1
Identifiers
Doi:
10.1080/01483919008049014
ISSN:
01483919
Study Approach
Quantitative