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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Insights into the population structure of Mycobacterium tuberculosis using spoligotyping and RD
Rio
in a southeastern Brazilian prison unit
Infection, Genetics and Evolution, Volume 26, Year 2014
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Description
Tuberculosis (TB) is still a serious public health problem, continuing to be an important threat for confined populations. We used spoligotyping to estimate the genotypic clades of Mycobacterium tuberculosis isolates from inmates in two blocks in a southeastern Brazilian prison unit, with TB incidence rate of 8185/100.000. The Latin American Mediterranean (LAM) clade is well represented in the country, and the LAM specific molecular markers, RDRio large sequence polymorphism and the SNP on the Rv3062 [ligB1212], were used to characterize spoligotype signatures from prison isolates. Typing of RDRio and ligB increase LAM clade from 66.7% (n=72/108) to 69.4% (n=75). The LAM2 SIT17 (n=23) and SIT179 (n=12) signatures comprised one third of all isolates, followed by Haarlem (11.5%, n=12), T (8.7%, n=9) and X (5.7%, n=6) clades. Strains with unknown signatures represented 5.5% (n=6), and four (3.7%) did not match any lineage. We observed RDRio among 64 (59.2%) isolates, and 54 (50%) were of the LAM clade. In particular, the LAM2/RDRio sub-lineage was significantly associated with clustering (p=0.02) and its frequency was higher (32%) when compared to that of the previous general TB cases in RJ (4.29%). Overall cluster frequency defined by spoligotyping/IS6110-RFLP was 62%. The two evolutionary markers helped to evaluate some LAM signature misconceptions and demonstrate that LAM2/RDRio was found with high frequency, hitherto being unnoticed. All these data, allied to high clustering, imply that public health measures to minimize the escalation of TB in prison is essential, and both spoligotyping as well as RDRio would be useful tools to monitor the effects of the measures with respect to M. tuberculosis lineage variation. © 2014 Elsevier B.V.
Authors & Co-Authors
Huber, Fé Dagmar
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Sánchez, Alexandra
Brazil, Rio de Janeiro
Tuberculosis Control Program and Coordination Management in Prison Health
Gomes, Harrison Magdinier
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Vasconcellos, Sidra E.G.
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Massari, Véronique
France, Paris
Institut Pierre Louis D'epidémiologie et de Santé Publique
France, Paris
Sorbonne Université
Barreto, Angela
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Cesconi, Vanderci
Brazil, Rio de Janeiro
Tuberculosis Control Program and Coordination Management in Prison Health
de Almeida Machado, Silvia Maria
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Gomgnimbou, Michel Kiréopori
France, Gif-sur-yvette
Université Paris-saclay
France, Paris
Cnrs Centre National de la Recherche Scientifique
Burkina Faso, Ouagadougou
Centre Muraz
Sola, Christophe Noel
France, Gif-sur-yvette
Université Paris-saclay
France, Paris
Cnrs Centre National de la Recherche Scientifique
Larouzé, Bernard
France, Paris
Institut Pierre Louis D'epidémiologie et de Santé Publique
France, Paris
Sorbonne Université
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Suffys, Philip Noel
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Saad, Maria Helena Féres
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Statistics
Citations: 13
Authors: 13
Affiliations: 7
Identifiers
Doi:
10.1016/j.meegid.2014.05.031
ISSN:
15671348
e-ISSN:
15677257
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study
Cohort Study