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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Evaluation of amino acid content and nutritional quality of transgenic soybean seeds with high-level tryptophan accumulation
Molecular Breeding, Volume 25, No. 2, Year 2010
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Description
Anthranilate synthase (AS) is a key regulatory enzyme in tryptophan (Trp) biosynthesis and is subject to feedback inhibition by Trp. The gene encoding a mutated feedback-resistant α subunit of rice AS (OASA1D) under the control of either a soybean glycinin gene promoter or the 35S promoter of cauliflower mosaic virus for seed-specific or constitutive expression, respectively, was introduced into soybean [Glycine max (L.) Merrill] by particle bombardment. A total of seven different transgenic lines that showed markedly increased accumulation of free Trp in their seeds were developed. The overproduction of free Trp was stably inherited in subsequent generations without any apparent detrimental effect on plant growth or reproduction. The total Trp content of transgenic seeds was also about twice that of nontransgenic seeds, whereas the amount of protein-bound Trp was not substantially affected by OASA1D expression. In spite of the marked increase in free Trp content, metabolic profiling by high-performance liquid chromatography coupled with mass spectrometry revealed little change in the amounts of other aromatic compounds in the transgenic seeds. We developed a rapid and feasible system based on farmed rainbow trout to evaluate the nutritional quality of a limited quantity of transgenic soybean seeds. Supplementation of fish food with OASA1D transgenic soybean seeds or with nontransgenic seeds plus crystalline Trp increased the growth rate of the farmed fish. These results indicate transformation with OASA1D is a reliable approach to improve the nutritional quality of soybean (or of other grain legumes) for human and animal food. © Springer Science+Business Media B.V. 2009.
Authors & Co-Authors
Ishimoto, Masao
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Sapporo
Hokkaido Agricultural Research Center, Naro
Japan, Hiroshima
Western Region Agricultural Research Center, Naro
Rahman, Shaikh Mizanur Mahbubur
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Hiroshima
Western Region Agricultural Research Center, Naro
Japan, Chikushino
Fukuoka Agricultural Research Center
Hanafy, Moemen S.
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Sapporo
Hokkaido Agricultural Research Center, Naro
Japan, Hiroshima
Western Region Agricultural Research Center, Naro
Egypt, Giza
National Research Centre
Khalafalla, Mutasim Mohamed
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Hiroshima
Western Region Agricultural Research Center, Naro
Sudan, Khartoum
Commission for Biotechnology and Genetic Engineering
El-Shemy, Hany A.
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Hiroshima
Western Region Agricultural Research Center, Naro
Egypt, Giza
Faculty of Agriculture
Nakamoto, Yumi
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Sapporo
Hokkaido Agricultural Research Center, Naro
Kita, Yohichi
Japan, Sapporo
Hokkaido Agricultural Research Center, Naro
Japan, Sapporo
Hokkaido University
Takanashi, Kojiro
Japan, Kyoto
Kyoto University
Matsuda, Fumio
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Wako
Riken
Murano, Yoshihiro
Japan, Tokyo
The Nisshin Oillio Group, Ltd.
Funabashi, Tomoko
Japan, Tokyo
The Nisshin Oillio Group, Ltd.
Miyagawa, Hisashi
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Kyoto
Kyoto University
Wakasa, Kyo
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Tokyo
Tokyo University of Agriculture
Statistics
Citations: 55
Authors: 13
Affiliations: 12
Identifiers
Doi:
10.1007/s11032-009-9334-3
ISSN:
13803743
Research Areas
Food Security
Genetics And Genomics
Noncommunicable Diseases