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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8
+
T cell responses
Veterinary Immunology and Immunopathology, Volume 130, No. 1-2, Year 2009
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Description
Immunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been identified but an effective delivery strategy that consistently induces protective CD8+ T cell responses remains to be developed. This study aimed to determine whether fusing Tat, a cell penetrating peptide (CPP) from HIV-1 TAT, to a CD8+ T cell target antigen from T. parva (Tp2) enhances the cytosolic delivery and subsequent stimulation of bovine CD8+ T cell responses in vitro. Using IFN-γ ELISpot and cytotoxicity assays, it was demonstrated that recombinant Tat-Tp2 fusion protein possessed a superior ability to access MHC-I processing and presentation pathway and to stimulate CD8+ T cell responses compared to recombinant Tp2 protein. Exposure of APC to Tat-Tp2 protein for only 30 min was sufficient for protein uptake and stimulation of CD8+ T cells. This work describes for the first time the utility of a CPP to enhance MHC-I presentation in a veterinary species and supports the evaluation of CPP fusion proteins in the induction of CD8+ T cell responses in vivo. © 2009 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Tinega, Alex N.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Kenya, Nairobi
Kenyatta University
Pellé, Roger
Kenya, Nairobi
International Livestock Research Institute Nairobi
Kang'A, Simon
United States, Rockville
J. Craig Venter Institute
Gicheru, Michael Muita
Kenya, Nairobi
Kenyatta University
Taracha, Evans L.N.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Nene, Vishvanath M.
United States, Rockville
J. Craig Venter Institute
Graham, Simon P.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Statistics
Citations: 7
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1016/j.vetimm.2009.01.008
ISSN:
01652427
Research Areas
Health System And Policy
Infectious Diseases