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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Analysis of polymorphic sites in the promoter of the nitric oxide synthase 2 gene
Biochemical and Biophysical Research Communications, Volume 335, No. 4, Year 2005
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Description
A point mutation (G → C) in the gene promoter for the human nitric oxide synthase (NOS) 2 at position -954 is associated with protection against severe Plasmodium falciparum malaria in Gabon. Carriers of this mutation show higher basal levels of nitric oxide production than wild type individuals. To obtain information about the possible binding transcription factors, nucleic proteins from the lung carcinoma cell line were enriched by affinity chromatography using DEAE-Sepharose and immobilized oligonucleotides derived from the promoter sequence. A mutational analysis was performed on 30 samples to detect polymorphisms in the NOS2 promoter region that contains important NF-κB sites. Three point mutations were identified in this region. In vitro studies with promoter constructs showed an altered expression of the marker gene depending on the promoter variant used. © 2005 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Coia, Valentina
Germany, Tubingen
Eberhard Karls Universität Tübingen
Italy, Rome
Sapienza Università Di Roma
Jüliger, Simone
Germany, Tubingen
Eberhard Karls Universität Tübingen
Mordmüller, Benjamin G.
Germany, Tubingen
Eberhard Karls Universität Tübingen
Destro Bisol, Giovanni Giovanni
Italy, Rome
Sapienza Università Di Roma
Kun, Juergen F.J.
Germany, Tubingen
Eberhard Karls Universität Tübingen
Statistics
Citations: 13
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/j.bbrc.2005.07.178
ISSN:
0006291X
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Locations
Gabon