Rubrobacter aplysinae sp. nov., isolated from the marine sponge Aplysina aerophoba

A Gram-stain-positive, non-spore-forming bacterium (strain RV113 T ) was isolated from the marine sponge Aplysina aerophoba. 16S rRNA gene sequence analysis showed that strain RV113 T belongs to the genusRubrobacter, and is related most closely to Rubrobacter bracarensisVF70612_S1 T (96.9 % similarity) and more distantly related (<93 %) to all other species of the genus Rubrobacter. The peptidoglycan diamino acid was lysine. Strain RV113 T exhibited a quinone system with menaquinone MK-8 as the predominant compound. The polar lipid profile of strain RV113 T consisted of the major compounds phosphatidylglycerol and two unidentified phosphoglycolipids. The major fatty acid was anteiso-C 17: 0 ω9c. These chemotaxonomic traits are in agreement with those of other species of the genus Rubrobacter. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of strain RV113 T from all recognized Rubrobacter species. Strain RV113 T is thus considered to represent a novel species, for which the name Rubrobacter aplysinae sp. nov. is proposed. The type strain is RV113 T (= DSM 27440 T = CECT 8425 T ) © 2014 IUMS.